159,117 results

1. PB-TAC-ERN

   Plasmid

   #80475

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TDP-43_CTD

   Plasmid

   #98669

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. modRNAc1-Cas9-2A-p53DD

   Plasmid

   #216389

   Purpose

   this vector can be used to make modRNA expressing Cas9 and p53DD

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

   Insert

   Cas9-2A-p53DD
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCHW101020

   Plasmid

   #222454

   Purpose

   Level 1, Position 2 containing 35S::CUP2-p65::NOS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   35S::CUP2-p65::NOS (CUP2 )
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFETCh_Donor (EMM0021)

   Plasmid

   #63934

   Purpose

   3X Flag with P2A-Neo for 3' tagging of endogenous proteins

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Glycine-Serine-3XFlag-P2A-NeoR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

   Plasmid

   #154473

   Purpose

   Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   SFFV

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_LacZ

   Plasmid

   #25893

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   LacZ
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. PLJR962

   Plasmid

   #115162

   Purpose

   Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

   Depositor

   Sarah Fortune

   Article

   Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

   Inserts

   Sth1 sgRNA scaffold
   Sth1 dCas9
   TetR
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-MMLVgag-3xNES-Cas9

   Plasmid

   #181752

   Purpose

   MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLV-Gag-3xNES-Cas9 (Spy)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-MBP-TEV

    Plasmid

    #69929

    Purpose

    bacterial expression of MBP-TEV n-terminal fusions

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    MBP and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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