We narrowed to 15,732 results for: LEA

1. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

   Plasmid

   #105803

   Purpose

   Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-FLAG; ORF2: TEV-mClover3

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLY54

   Plasmid

   #130923

   Purpose

   A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag and pspFΔHTH::λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Anderson promoter: J23106, PpspA-LEA2B2, and Ptet

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. dopamine beta hydroxylase (dbh)_L (OZ525)

   Plasmid

   #27200

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting dbh (dbh Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75243

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Ikaros (IKZF1 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Swachmann-Bodian-Diamond Syndrome Gene, sbds_R (OZ546)

   Plasmid

   #28071

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting Swachmann-Bodian-Diamond Syndrome Gene, sbds (sbds Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

6. t1-400

   Plasmid

   #166577

   Purpose

   For expression of human talin head (residues 1-400) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

   Depositor

   Vesa Hytönen

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

   Insert

   Tln1 Head (1-400) (TLN1 Human)
   

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   residues 1-400 only

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Swachmann-Bodian-Diamond Syndrome Gene, sbds_L (OZ545)

   Plasmid

   #28070

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting Swachmann-Bodian-Diamond Syndrome Gene, sbds (sbds Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pHAGE2-TetOminiCMV-KS

   Plasmid

   #136617

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Klf4-IRES-Sox2 (Klf4, Sox2 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. cftr_R (OZ518)

   Plasmid

   #27193

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting cftr (cftr Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

10. cdkn1a(p21)_L (OZ555)

    Plasmid

    #28080

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting cdkn1a(p21) (cdkn1a Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

11. cdkn1a(p21)_R (OZ556)

    Plasmid

    #28081

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting cdkn1a(p21) (cdkn1a Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

12. Atg7_L (OZ557)

    Plasmid

    #28082

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting Atg7 (atg7 Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

13. Atg7_R (OZ558)

    Plasmid

    #28083

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting Atg7 (atg7 Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

14. cftr_L (OZ517)

    Plasmid

    #27192

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting cftr (cftr Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pDEST26 Ago2 5A

    Plasmid

    #60651

    Purpose

    Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Tags

    6xHis (N-termi tag)

    Expression

    Mammalian

    Mutation

    mutated five serines to alanines

    Promoter

    CMV

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. atp2c1_L (OZ567)

    Plasmid

    #33381

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting atp2c1 (atp2c1 Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

17. dopamine beta hydroxylase (dbh)_R (OZ526)

    Plasmid

    #27201

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting dbh (dbh Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75242

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. apoeb (apolipoprotein Eb)_L (OZ529)

    Plasmid

    #27206

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting apoeb (apoeb Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. apoeb (apolipoprotein Eb)_R (OZ530)

    Plasmid

    #27207

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting apoeb (apoeb Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only