We narrowed to 17,821 results for: Por
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Plasmid#208722PurposeExpresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2m
UseAAVPromoterEF1aAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s-T78H
Plasmid#100021PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78HDepositorInsertsfMatryoshCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmNanoLuc
Plasmid#182969PurposeStably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmNanoLuc
ExpressionMammalianMutationN/AAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_hph
Plasmid#184919PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)
Plasmid#100743PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice formDepositorAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
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EGFP-uTEV1-MAVS-mTA4
Plasmid#171002PurposeHiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC39A14
Plasmid#132105PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC39A14 (SLC39A14 Human)
ExpressionMammalianAvailable SinceDec. 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Hy_pMT Flag-Hel25E K91N
Plasmid#110121PurposeExpresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activityDepositorInsertHel25E (Hel25E Fly)
TagsFlagExpressionInsectMutationK91N mutationPromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E D193E
Plasmid#110120PurposeExpresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinityDepositorInsertHel25E (Hel25E Fly)
TagsFlagExpressionInsectMutationD193E mutationPromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC7A3
Plasmid#132301PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A3 (SLC7A3 Human)
ExpressionMammalianAvailable SinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
FL MALT1 Catalytic Mutant in pET29b-Strep
Plasmid#48969PurposeExpresses Full length catalytic mutant MALT1 in e.coliDepositorInsertMALT1 isoform A (MALT1 Human)
TagsHis and StrepExpressionBacterialMutationCatalytic Mutant C464APromoterT7Available SinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-Crry-T2A-GFP
Plasmid#205452Purposelentiviral plasmid for expression of mouse CrryDepositorAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_32B
Plasmid#75011PurposePortion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_32B
ExpressionBacterialPromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_27B
Plasmid#75010PurposePortion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_27B
ExpressionBacterialPromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF17 tail 400-1038
Plasmid#134617PurposeMammalian expression of mouse KIF1A 400-1038DepositorInsertKIF17 (Kif17 Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 399 N-terminal residues.PromoterChicken Beta actinAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-F-API5
Plasmid#157662PurposeExpresses API5 in mammalian cellDepositorAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GSDMD_NoCys-Myc
Plasmid#201169PurposeExpression of GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D (Gsdmd Mouse)
UseRetroviralTagsMycMutationC39A, C57A, C77A, C122A, C265A, C192APromoterMESVAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 LNG-6MT
Plasmid#41738DepositorInsertNotch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
Tags6xMycExpressionMammalianMutationContains murine Notch-1 leader peptide (aa 1-23) …Available SinceJan. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28b-API5
Plasmid#157655PurposeExpresses API5 in E.coli cellDepositorAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only