1. p426 46Q GPD

   Plasmid

   #1182

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 46Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. AAV-CAG-FLPX-rc [ChrimsonR-GFP]

   Plasmid

   #118295

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   CAG

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZH_mPP7cp_mCerulean3

   Plasmid

   #118262

   Purpose

   Low-noise, tetracycline inducible expression of mPP7cp-mCerulean3

   Depositor

   Zach Hensel

   Insert

   mPP7cp-mCerulean3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBSV2_P0526-mCherryBb

   Plasmid

   #118241

   Purpose

   Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0526 promoter driven mCherry

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   bb0526 promoter driven mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBSV2_PresT-mCherryBb

   Plasmid

   #118238

   Purpose

   Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   resT promoter driven mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZH_tdPP7cp_mCerulean3

   Plasmid

   #118263

   Purpose

   Low-noise, tetracycline inducible expression of tdPP7cp-mCerulean3

   Depositor

   Zach Hensel

   Insert

   tdPP7cp-mCerulean3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE

   Plasmid

   #118279

   Purpose

   Expresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cells

   Depositor

   Dietmar Schmitz

   Article

   Wozny et al Front Cell Neurosci. 2018 Oct 2;12:337.

   Inserts

   mRuby2
   ChR2(H134R)
   

   Use

   AAV and Cre/Lox

   Tags

   SV40 NLS and YFP

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZH_mPP7cp_mCherry

   Plasmid

   #118266

   Purpose

   Low-noise, tetracycline inducible expression of mPP7cp-mCherry

   Depositor

   Zach Hensel

   Insert

   mPP7cp-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. WT MetNIQ in pBAD

   Plasmid

   #118253

   Purpose

   Wild Type Methionine Binding Protein and Methionine ABC Transporter in pBAD Vector for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (metN E. coli)
   MetI (metI E. coli)
   MetQ (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Glutamic Acid (Glu) 295 to Alanine (Ala)

   Promoter

   None and araBAD Promoter

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FLAG-OTUB1 T134R,C91S-pcDNA3.1

   Plasmid

   #118212

   Purpose

   Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed cysteine 91 to serine and threonine 134 t…

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBSV2_P0031-mCherryBb

    Plasmid

    #118240

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0031 promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    bb0031 promoter driven mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. N229A (EcQ) MetNIQ in pBAD

    Plasmid

    #118257

    Purpose

    N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (N229A) (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Asparagine (Asn) 229 to Alanine (Ala) and…

    Promoter

    None and araBAD Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. N295A (EcN) MetNIQ in pBAD

    Plasmid

    #118256

    Purpose

    N295A Mutation of MetN in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Asparagine (Asn) 295 to Alanine (Ala)

    Promoter

    None and araBAD Promotoer

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

    Plasmid

    #118260

    Purpose

    N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (Y160A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmKO-AF8

    Plasmid

    #118224

    Purpose

    Kusabira- Orange-CENP-A expression plasmid

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Mutat Res. 2008 Nov 17;657(1):56-62. doi

    Insert

    CENP-A (CENPA Human)
    

    Use

    Tags

    Kusabira orange

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. WT MetNI in pBAD

    Plasmid

    #118254

    Purpose

    Wild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN Escherichia coli)
    MetI (metI Escherichia coli)
    

    Use

    Tags

    10x His Tag and Enterokinase Cleavage Site

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose Promoter and None

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-ERK2 del174-177

    Plasmid

    #118297

    Purpose

    Expression of Rat ERK2

    Depositor

    Philip Stork

    Article

    Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

    Insert

    ERK2 del174-177 (Mapk1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion 174aa-177aa

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-DYRK4-2A-mCherry

    Plasmid

    #118289

    Purpose

    Expresses mouse DYRK4 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (Dyrk4 Mouse)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTXB1-xlH2B(1-114)_K114A

    Plasmid

    #118217

    Purpose

    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    xlH2B(1-114)
    

    Use

    Tags

    Mxe-GyrA intein fused to chitin binding domain

    Expression

    Bacterial

    Mutation

    Lysine 114 to alanine

    Promoter

    T7

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZH_tdPP7cp_ECFP

    Plasmid

    #118265

    Purpose

    Low-noise, tetracycline inducible expression of tdPP7cp-ECFP

    Depositor

    Zach Hensel

    Insert

    tdPP7cp-ECFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZH_mPP7cp_ECFP

    Plasmid

    #118264

    Purpose

    Low-noise, tetracycline inducible expression of mPP7cp-ECFP

    Depositor

    Zach Hensel

    Insert

    mPP7cp-ECFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge