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pEGFP-N1-delta30-TFEB

Plasmid

#44445

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

GFP

Expression

Mammalian

Mutation

Deleted amino acids 1-30; H32D

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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PS Intein Bid

Plasmid

#242024

Purpose

Blue-light-induced protein cleavage: apoptosis via Bid activation.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

PS Intein Bid

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PS Intein/C1A Bid

Plasmid

#242025

Purpose

PS Intein Bid construct containing the C1A mutation in gp41-1, which abolishes its autocatalytic splicing activity.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

PS Intein/C1A Bid

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHPHS309

Plasmid

#228230

Purpose

GFP dropout backbone for sgRNA library cloning

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-PS Intein

Plasmid

#242020

Purpose

AAV version of PS Intein palsmid.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

PS Intein

Use

Adenoviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pegfp-N1-TFEB-S3A,R4A

Plasmid

#44446

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed both Serine 3 and Arginine 4 to Alanine

Promoter

CMV

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pSYC-97

Plasmid

#31565

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

pCS4+-NLS-EGFP-P2A-mCherry-CAAX

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRS415_pGal-nCas9(H840A)

Plasmid

#79616

Purpose

Expresses nCas9(H840A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

H840A for nickase Cas9

Promoter

pGal1

Available Since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-BpBBS

Plasmid

#199467

Purpose

Arabinose-induced expression of His-tagged biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in E. coli.

Depositor

Vladislav Verkhusha

Article

Manoilov et al J Mol Biol. 2022 Jan 30;434(2):167359. d

Insert

BpBBS

Tags

6xHis

Expression

Bacterial

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-SH3

Plasmid

#79613

Purpose

Expresses dCas9-SH3 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

SH3 domain

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGC684

Plasmid

#107022

Purpose

GFP reporter of lag-2 driven by 1 kb of promoter, which lackes both DBS and Cons motifs

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Tags

GFP

Expression

Worm

Promoter

lag-2

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGC682

Plasmid

#107020

Purpose

GFP reporter of lag-2 driven by 1 kb of promoter, which lackes DBS motif

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Tags

GFP

Expression

Worm

Promoter

lag-2

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-TFEB

Plasmid

#38119

Purpose

Mammalian expression of Transcription factor EB fused to EGFP

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-TFE3

Plasmid

#38120

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFE3 (TFE3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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WT GBA pcDNA3.1

Plasmid

#188580

Purpose

Ectopic expression of wild-type GBA

Depositor

Anthony Schapira

Article

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-A

Plasmid

#38132

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-A (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-tdTomatof

Plasmid

#104060

Purpose

Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

tdTomato

Use

AAV

Promoter

hSyn

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mNeonGreen

Plasmid

#99134

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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N370S GBA pcDNA3.1

Plasmid

#188583

Purpose

Ectopic expression N370S GBA

Depositor

Anthony Schapira

Article

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Expression

Mammalian

Mutation

N370S

Promoter

CMV

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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