We narrowed to 313 results for: Ky

1. pEGFP-N1-delta30-TFEB

   Plasmid

   #44445

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-30; H32D

   Promoter

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS415_pGal-nCas9(D10A)

   Plasmid

   #79618

   Purpose

   Expresses nCas9(D10A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   D10A for nickase Cas9

   Promoter

   pGal1

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pegfp-N1-TFEB-S3A,R4A

   Plasmid

   #44446

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed both Serine 3 and Arginine 4 to Alanine

   Promoter

   CMV

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSYC-97

   Plasmid

   #31565

   Depositor

   Seok-Yong Choi

   Article

   Kim et al PLoS One. 2011 . 6(4):e18556.

   Insert

   pCS4+-NLS-EGFP-P2A-mCherry-CAAX
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS415_pGal-nCas9(H840A)

   Plasmid

   #79616

   Purpose

   Expresses nCas9(H840A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H840A for nickase Cas9

   Promoter

   pGal1

   Available since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBAD-BpBBS

   Plasmid

   #199467

   Purpose

   Arabinose-induced expression of His-tagged biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in E. coli.

   Depositor

   Vladislav Verkhusha

   Article

   Manoilov et al J Mol Biol. 2022 Jan 30;434(2):167359. d

   Insert

   BpBBS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS315e_pGal-dCas9-SH3

   Plasmid

   #79613

   Purpose

   Expresses dCas9-SH3 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   SH3 domain

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHPHS927

   Plasmid

   #228238

   Purpose

   Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin reporter for integration into Bxb1 attP_GA landing pad

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

   Inserts

   mCherry
   Beta globin
   

   Use

   Tags

   3xFLAG, HA, and PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV and none

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHPHS800

   Plasmid

   #228240

   Purpose

   Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

   Insert

   Cas9
   

   Use

   Tags

   Hygromycin phosphotransferase

   Expression

   Mammalian

   Mutation

   Promoter

   none

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGC684

    Plasmid

    #107022

    Purpose

    GFP reporter of lag-2 driven by 1 kb of promoter, which lackes both DBS and Cons motifs

    Depositor

    Jane Hubbard

    Article

    Pekar et al Development. 2017 Aug 15;144(16):2896-29

    Insert

    GFP-PH
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    lag-2

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGC682

    Plasmid

    #107020

    Purpose

    GFP reporter of lag-2 driven by 1 kb of promoter, which lackes DBS motif

    Depositor

    Jane Hubbard

    Article

    Pekar et al Development. 2017 Aug 15;144(16):2896-29

    Insert

    GFP-PH
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    lag-2

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2+ GFP-2xESP3I-IRES-mCherry

    Plasmid

    #230996

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Type

    Empty backbone

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-mTH-GFP

    Plasmid

    #99128

    Purpose

    An AAV genome that expresses GFP from the mouse TH promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mTH

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-N1-TFEB

    Plasmid

    #38119

    Purpose

    Mammalian expression of Transcription factor EB fused to EGFP

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFEB (TFEB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. WT GBA pcDNA3.1

    Plasmid

    #188580

    Purpose

    Ectopic expression of wild-type GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-N1-TFE3

    Plasmid

    #38120

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFE3 (TFE3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-TRE-eYFP

    Plasmid

    #104056

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein eYFP

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. N370S GBA pcDNA3.1

    Plasmid

    #188583

    Purpose

    Ectopic expression N370S GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N370S

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L444P GBA pcDNA3.1

    Plasmid

    #188582

    Purpose

    Ectopic expression L444P GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L444P

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCX-V5-H7N9-NS1

    Plasmid

    #99022

    Purpose

    Mammalian expression of NS1

    Depositor

    Honglin Chen

    Article

    Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

    Insert

    NS1
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge