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CAG sHRPa-NRX

Plasmid

#73143

Purpose

Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-NRX

Use

Tags

2x HA tag and Flag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RPA2

Plasmid

#22893

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

RPA2 (RPA2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP-RPA70

Plasmid

#164231

Purpose

retroviral plasmid for GFP-RPA70

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

RPA70 (RPA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA-32Asp8

Plasmid

#102617

Purpose

Expression of human RPA with aspartic acid substituted phosphorylation domain

Depositor

Marc Wold

Article

Binz et al J Biol Chem. 2003 Sep 12;278(37):35584-9

Inserts

RPA1 (RPA1 Human)
RPA3 (RPA3 )
RPA2 (RPA2 )

Use

Tags

Expression

Bacterial

Mutation

with aspartic acid substituted phosphorylation do…

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA-32Ala9

Plasmid

#102616

Purpose

Expression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).

Depositor

Marc Wold

Inserts

RPA1 (RPA1 Human)
RPA3 (RPA3 )
RPA2 (RPA2 )

Use

Tags

Expression

Bacterial

Mutation

with an alanine substituted phosphorylation domain

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJY-RpABE-PDS3_IMS

Plasmid

#112875

Purpose

binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

Depositor

Jae-Young Yun, Jin-Soo Kim

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA targeting PDS3

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay sHRPa-KDEL

Plasmid

#73149

Purpose

Large fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-KDEL

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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Bsrs103-RpaR-LasI

Plasmid

#85160

Purpose

Constitutively expresses RpaR and inducibly expresses LasI

Depositor

Jeff Hasty

Article

Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

Insert

RpaR, LasI

Use

Tags

Expression

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCI.neo-rPAM1-M314I

Plasmid

#145802

Purpose

expresses catalytically inactive rat PAM-1 in mammalian cells

Depositor

Richard Mains, Betty Eipper

Article

Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

Insert

rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)

Use

Tags

Expression

Mammalian

Mutation

changed methionine 314 to isoleucine to inactivat…

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRPa-iSL(MCS1+2)

Plasmid

#69244

Purpose

Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

Depositor

David Horn

Article

Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

Type

Empty backbone

Use

Expression in trypanosoma brucei

Tags

Expression

Mutation

Promoter

RRNA promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Trpa1b-FLAG

Plasmid

#106403

Purpose

Mammalian expression vector containing zebrafish Trpa1b with C-terminal FLAG tag.

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Insert

Trpa1b-FLAG (trpa1b Zebrafish)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA (KmR)

Plasmid

#102321

Purpose

drives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ kanamycin resistance casette)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

-

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJY-RpABE-FT_INF

Plasmid

#112874

Purpose

binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis

Depositor

Jae-Young Yun, Jin-Soo Kim

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA targeting FT

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Ptrc::RpaA D53A

Plasmid

#105229

Purpose

inducible expression of unphosphorylatable variant of RpaA (D53A)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

rpaA driven by Ptrc promoter

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

D53 mutated to A

Promoter

ptrc

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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sg_Snrpa_i2-ipUSEPR-TR657

Plasmid

#228923

Purpose

Knockdown of Snrpa in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Snrpa_i2 (Snrpa Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Snrpa_i4-ipUSEPR-TR657

Plasmid

#228924

Purpose

Knockdown of Snrpa in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Snrpa_i4 (Snrpa Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RPA32-mVen

Plasmid

#211388

Purpose

mammalian expression of human RPA32 C-terminally fused to mVenus

Depositor

Helmut Pospiech

Insert

RPA32 (RPA2 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RPA32-mCer

Plasmid

#211387

Purpose

mammalian expression of human RPA32 C-terminally fused to mCerulean

Depositor

Helmut Pospiech

Insert

RPA32 (RPA2 Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgSNRPA guide 1

Plasmid

#193598

Purpose

SNRPA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgSNRPA guide 1 (SNRPA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgSNRPA guide 2

Plasmid

#193599

Purpose

SNRPA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgSNRPA guide 2 (SNRPA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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