We narrowed to 3,415 results for: guide rna expression plasmid

1. LentiGuide-Puro-NCOA7g1 (BB22)

   Plasmid

   #139457

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiGuide-Puro-NCOA7g2 (BB23)

   Plasmid

   #139458

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LentiGuide-Puro-NCOA7g3 (BB24)

   Plasmid

   #139459

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LentiGuide-Neo-NCOA7g1 (BB34)

   Plasmid

   #139452

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LentiGuide-Neo-NCOA7g3 (BB36)

   Plasmid

   #139454

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LentiGuide-Neo-NCOA7g2 (BB35)

   Plasmid

   #139453

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GG-EBNA-MIR302-7guides-PGK-Puro

   Plasmid

   #201960

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

   Depositor

   Timo Otonkoski

   Article

   Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

   Insert

   MIR302-7g-PGK-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G

   Plasmid

   #157982

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Tetra-8A8G)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiP927-pX330-Chrna6

   Plasmid

   #181765

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Chrna6 guide
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pgRNA_AT

    Plasmid

    #196243

    Purpose

    Backbone plasmid for cloning sgRNA

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pgRNA-bacteria

    Plasmid

    #44251

    Purpose

    Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA (bacteria)
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119 (SpeI)

    Available Since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Noodles Plasmid

    Plasmid

    #212613

    Purpose

    An all-in-one system for on-target efficiency analysis of CRISPR guide RNAs

    Depositor

    Ilya Vinnikov

    Article

    Lin et al MethodsX. 2023 Nov 15;12:102481. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Luciferase

    Expression

    Mammalian

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pgRNA-humanized

    Plasmid

    #44248

    Purpose

    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSPgRNA

    Plasmid

    #47108

    Purpose

    Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    SPgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC119-gRNA

    Plasmid

    #52255

    Purpose

    Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
    

    Use

    CRISPR; Plant expression

    Promoter

    Arabidopsis U6 polymerase III promoter

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pgRNA-R-gfp

    Plasmid

    #220927

    Purpose

    Expresses gfp using J23119 promoter and pgRNA_AT backbone

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    superfolder GFP
    

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR_AtU6p_BsaI_gRNA

    Plasmid

    #225987

    Purpose

    To make Gateway entry clone of CRISPR guide RNA under AtU6p

    Depositor

    Imran Siddiqi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pHelper_ShCAST_sgRNA

    Plasmid

    #127921

    Purpose

    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR_AtU6p_BsaI_ccdb_gRNA

    Plasmid

    #225986

    Purpose

    To make Gateway entry clone of CRISPR guide RNA under AtU6p

    Depositor

    Imran Siddiqi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAdSh.U6.gRNAGFP

    Plasmid

    #58255

    Purpose

    U6 promoter-driven single guide RNA complementary to eGFP

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAGFP cassette
    

    Use

    Adenoviral and CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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