Skip to main content
Addgene
Showing: 61 - 80 of 501 results

  2. pSLQ10871

    Plasmid
    #183962
    Purpose
    U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    dCas12a(wildtype)
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG and U6
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183962-plasmid-map-sequence-id-363117

    Enlarge

  4. pSLQ10878

    Plasmid
    #183964
    Purpose
    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183964-plasmid-map-sequence-id-363120

    Enlarge

  5. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

    Enlarge

  6. pGL004

    Plasmid
    #215336
    Purpose
    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)
    Depositor
    Gene-Wei Li
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    hyper-pSpank
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215336-plasmid-map-sequence-id-426101

    Enlarge

  12. p09008_AF1-4+GFP5-7

    Plasmid
    #173715
    Purpose
    Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.
    Depositor
    Johannes Kabisch
    Article
    Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8
    Inserts
    hybrid GFP
    mKate2
    Use
    E. coli cell free protein synthesis extracts
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    173715-plasmid-map-sequence-id-361303

    Enlarge

  14. pMT-ADARcd-E488Q-V5

    Plasmid
    #139686
    Purpose
    Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5
    Depositor
    Michael Rosbash
    Article
    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126
    Insert
    Adenosine deaminase acting on RNA (Adar Fly)
    Use
    Tags
    V5
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139686-plasmid-map-sequence-id-274450

    Enlarge

Showing: 61 - 80 of 501 results