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Plasmid#196879PurposeNeuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.DepositorInsertNedd1-mOrange2-rCM1 (Nedd1 Rat)
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD
Plasmid#196894PurposeNeuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmidsDepositorInsertN-gTBD-mOrange2 (NEDD1 Human)
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD
Plasmid#196881PurposeNeuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.DepositorInsertmOrange2-Cdk5rap2-CTD
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-128-425-Akap9
Plasmid#196897PurposeNeuron-specific expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to 2xHA-tags.DepositorInsert2xHA-Akap9(128-425) (Akap9 Rat)
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA 3.1 Flag-rat-PTP1B
Plasmid#35988DepositorInsertprotein tyrosine phosphatase 1B (Ptpn1 Rat)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisA-rat-PTP1B
Plasmid#35987DepositorInsertprotein tyrosine phosphatase 1B (Ptpn1 Rat)
UseTagsHis and Xpress Epitope TagExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR-li-p33_RS-CLIP
Plasmid#104634PurposeHuman Invariant chain with a CLIP replacement with in frame restriction sites (KpnI/XhoI)DepositorInsertmodified Invariant chain p33 (CD74 Human)
UseEntry vector for gateway systemTagsnoneExpressionMutationCLIP domain was replaced by 2 restriction sitesPromoterAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIL2RmCherry
Plasmid#105317PurposeTo visualize membrane dynamicsDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpyB
Plasmid#220186PurposesgRNA expression vector - pBG35 promoterDepositorInsertpBG35 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpBG35Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-empty
Plasmid#220203Purposemini-Tn7 cloning vector - empty backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpy
Plasmid#220184PurposesgRNA expression vector - promoterless backboneDepositorInsertpromoterless backbone
UseCRISPRTagsExpressionBacterialMutationPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyA
Plasmid#220185PurposesgRNA expression vector - pEM7 promoterDepositorInsertpEM7 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpEM7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyC
Plasmid#220187PurposesgRNA expression vector - pBG42 promoterDepositorInsertpBG42 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpBG42Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyD
Plasmid#220188PurposesgRNA expression vector - pBG28 promoterDepositorInsertpBG28 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpBG28Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-lifeact-mAPPLE
Plasmid#89820PurposemAPPLE labeled fluorescent actinDepositorInsertlifeact-mAPPLE
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-MLC2 WT
Plasmid#195235PurposeMammalian expression of GFP tagged MLC2. Wild-typeDepositorInsertMLC2 (MYL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyC-GFP
Plasmid#220191PurposesgRNA expression vector - pBG42 promoter with GFP in sgRNA siteDepositorInsertpBG42 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyA-GFP
Plasmid#220189PurposesgRNA expression vector - pEM7 promoter with GFP in sgRNA siteDepositorInsertpEM7 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpEM7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyB-GFP
Plasmid#220190PurposesgRNA expression vector - pBG35 promoter with GFP in sgRNA siteDepositorInsertpBG35 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyD-GFP
Plasmid#220192PurposesgRNA expression vector - pBG28 promoter with GFP in sgRNA siteDepositorInsertpBG28 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG28Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only