Addgene

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pBigTB-FlpERT2

Plasmid

#149435

Purpose

tamoxifen-inducible Flp recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Flp-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBICD2-TUG

Plasmid

#52873

Purpose

retroviral expression of TUG

Depositor

Jonathan Bogan

Article

Bogan et al Nature. 2003 Oct 16;425(6959):727-33.

Insert

TUG (Aspscr1 Mouse)

Use

Retroviral

Tags

IRES-CD2 (mouse)

Expression

Mammalian

Mutation

Promoter

retrovirus LTR

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pBIP1::eYFP

Plasmid

#135231

Purpose

UPR signaling reporter (upregulates eYFP expression under UPR conditions)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

pBiP1

Use

Tags

eYFP

Expression

Plant

Mutation

Promoter

BiP1

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBIND-HBSP

Plasmid

#53113

Purpose

Expression of GAL4BD-tagged Hepatitis B splicing protein

Depositor

Xu Lin

Article

Chen et al BMC Cancer. 2014 Apr 23;14:282. doi: 10.

Insert

HBSP

Use

Tags

GAL4BD

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA5_FRB-pBirA-HA

Plasmid

#83415

Purpose

proximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

Insert

FRB-pBirA-HA (MTOR Human, Synthetic)

Use

Tags

HA and pBirA

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBiex1-SYNAC

Plasmid

#82710

Purpose

Synthetic soluble adenylyl cyclase FRET sensor and cAMP generator

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al Mol Pharmacol. 2016 Apr;89(4):407-12. do

Insert

SYNAC (ADCY2, ADCY5 Human)

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Bacterial and Insect

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG1a-PAF1CdeltaWDR61

Plasmid

#231745

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Inserts

CTR9 (CTR9 Human)
CDC73 (CDC73 Human)
PAF1 (PAF1 Human)
LEO1 (LEO1 Human)

Use

Tags

3C-Strep(II) and Flag-3C

Expression

Insect

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a-PAF1CdeltaCDC73

Plasmid

#231746

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Inserts

CTR9 (CTR9 Human)
PAF1 (PAF1 Human)
LEO1 (LEO1 Human)
WDR61 (SKIC8 Human)

Use

Tags

3C-Strep(II)

Expression

Insect

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a-PAF1CdeltaLEO1

Plasmid

#231747

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Inserts

CTR9 (CTR9 Human)
CDC73 (CDC73 Human)
PAF1 (PAF1 Human)
WDR61 (SKIC8 Human)

Use

Tags

3C-Strep(II) and Flag-3C

Expression

Insect

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook3_FTS_FHIP1B

Plasmid

#222299

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK3 and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Inserts

HOOK3 (HOOK3 Human)
FTS (AKTIP Human)
FHIP1B (FHIP1B Human)

Use

Tags

StrepII-Psc

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook2_FTS_FHIP2A

Plasmid

#222302

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector

Depositor

Andrew Carter

Inserts

HOOK2 (HOOK2 Human)
FTS (AKTIP Human)
FHIP2A (FHIP2A Human)

Use

Tags

StrepII-Psc

Expression

Insect

Mutation

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBICI_high ori

Plasmid

#179273

Purpose

Expresses CuCBP and BaSP in E. coli BL21(DE3)

Depositor

Bernd Nidetzky

Article

Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Mutation

Promoter

T7 lacO

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSicoR-CENPBi2

Plasmid

#59619

Purpose

Expression of shRNA against human CENPB

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

CENPB (CENPB Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pBI-MCS-EYFP

Plasmid

#58855

Purpose

The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

enhanced yellow fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pBITE-CD19-CD3

Plasmid

#190677

Purpose

Encodes a modularized form of HD37-OKT3 bispecific antibody

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Modularized-HD37-modularized-OKT3-P2A-NeonGreen

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBI121-GFP-RmMYB108

Plasmid

#173181

Purpose

Expresses Rosa multiflora MYB108 for subcellular localization in plants

Depositor

Daidi Che

Article

Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

Insert

RmMYB108

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_DNAI1:1-699

Plasmid

#211275

Purpose

Mammalian expression of full-length human DNAI1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DNAI1:M1-T699 (DNAI1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

A8S Y202C P495Q annotated sequence conflicts

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBI-EGFP-MnSOD

Plasmid

#16612

Depositor

Bert Vogelstein

Article

Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

Insert

MnSOD (SOD2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_CORO1A:1-461

Plasmid

#211111

Purpose

Mammalian expression of full-length human CORO1A. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1A:M1-K461 (CORO1A Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN155(I152L)

Plasmid

#27097

Depositor

Chang-Deng Hu

Article

Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

Insert

VN155(I152L)

Use

Tags

Myc

Expression

Mammalian

Mutation

I152L

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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