We narrowed to 1,605 results for: prep

1. pRepair-MPP8_mAID-P2A-BFP

   Plasmid

   #175036

   Purpose

   Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

   Depositor

   Kristian Helin

   Article

   Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

   Insert

   mAID-P2A-BFP
   

   Use

   Hdr donor template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG426-GAL-Prepro-3x-Adan

   Plasmid

   #181733

   Purpose

   Galactose inducible expression of Prepro-3x-Adan

   Depositor

   Alejandro Chavez

   Insert

   Prepro-3x-Adan
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTricHis-ILY-prepore-charge+

   Plasmid

   #160522

   Purpose

   Expression of inserted ILY variant

   Depositor

   Doryen Bubeck

   Article

   Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

   Insert

   ILY-prepore-charge+
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   I104C, G244C, Y336K, L340K

   Promoter

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTricHis-ILY-prepore-N342C

   Plasmid

   #160521

   Purpose

   Expression of inserted ILY variant

   Depositor

   Doryen Bubeck

   Article

   Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

   Insert

   ILY-prepore-N342C
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   I104C, G244C, N342C

   Promoter

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTricHis-ILY-prepore-L340C

   Plasmid

   #160520

   Purpose

   Expression of inserted ILY variant

   Depositor

   Doryen Bubeck

   Article

   Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

   Insert

   ILY-prepore-L340C
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   I104C, G244C, L340C

   Promoter

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-fDIO-mCherry-WPREpA

   Plasmid

   #177328

   Purpose

   Control vector for Flp-dependent DREADD constructs

   Depositor

   Ulrik Gether

   Insert

   mCherry
   

   Use

   AAV; Flp-frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin-1

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pREP-8xARE-GFP-SV40-BFP

   Plasmid

   #134910

   Purpose

   NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Inserts

   8xARE- minimal promoter - GFP
   TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   8xARE - minimal promoter and SV40

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC446 - pAAV EF1a hPREP(S554A)

   Plasmid

   #59968

   Purpose

   An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   Prolyl oligopeptidase (PREP Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S554A

   Promoter

   EF1a

   Available since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG426-GAL-Prepro-3x-Beta-Amyloid

   Plasmid

   #181730

   Purpose

   Galactose inducible expression of Prepro-3x-Beta-Amyloid

   Depositor

   Alejandro Chavez

   Insert

   Prepro-3x-Beta-Amyloid
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid

    #154868

    Purpose

    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

    Depositor

    Ulrik Gether

    Has Service

    AAV Retrograde and AAV8

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin-1

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

    Plasmid

    #84469

    Purpose

    pAAV-RAM-EGFP

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

    Plasmid

    #84471

    Purpose

    pAAV-RAM-ChR2

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    Channelrhodopsin 2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    H134R

    Promoter

    TRE

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA

    Plasmid

    #154867

    Purpose

    Flp-dependent hM4D(Gi) DREADD-mCherry

    Depositor

    Ulrik Gether

    Article

    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

    Has Service

    AAV Retrograde and AAV8

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin-1

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

    Plasmid

    #84472

    Purpose

    pAAV-RAM-ArchT

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    ArchT
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-fDIO-rM3D(Gs)-mCherry-WPREpA

    Plasmid

    #154869

    Purpose

    Human synapsin-1 promoter; Flp-dependent rM3D(Gs) DREADD-mCherry

    Depositor

    Ulrik Gether

    Insert

    rM3D(Gs)
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin-1

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

    Plasmid

    #225438

    Purpose

    Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

    Depositor

    Diego Orzaez

    Insert

    CBS-FRT-OCS-BeYDVRepRepA-tNos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA

    Plasmid

    #84468

    Purpose

    pAAV-CRAM-tdTomato

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    T230I (please see depositor comments below)

    Promoter

    TRE

    Available since

    Dec. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA

    Plasmid

    #84474

    Purpose

    pAAV-RAM-NLS-mKate2

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    mKate2
    

    Use

    AAV

    Tags

    Nuclear localization signal of SV40 large T antig…

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-fDIO-HA-KORD-IRES-mCitrine-WPREpA

    Plasmid

    #154871

    Purpose

    Human synapsin-1 promoter; Flp-dependent KORD-IRES-mCitrine

    Depositor

    Ulrik Gether

    Insert

    KORD-IRES-mCitrine
    

    Use

    AAV; Flp-frt

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin-1

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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