-
Plasmid#154058PurposeLevel 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosTDepositorInsertLoxP-flanked GUS and TtNAM-B1
UseSynthetic BiologyTagsExpressionBacterialMutationThe TtNAM-B1 gene sequence was domesticated to re…PromoterZmUbiAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCVD002
Plasmid#63688Purposefor use in sequencing and mapping of the V. cholerae enterotoxin genesDepositorInsertenterotoxin genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pME-mKate2-KDEL-pA
Plasmid#109546PurposeMultisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)DepositorInsertmKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPT-mCherry
Plasmid#190605PurposeObtained by cloning 5 stop codons in place of the EGFP Kozak sequence in pWPT-/mEGFP-1T-IRES-mCherryDepositorInsertsmEGFP
mCherry
UseLentiviralTagsExpressionMutationEGFP Kozak sequence has been replaced with a stre…PromoterEIF1-shortAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-EGFP
Plasmid#48348PurposeContributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEGFP
UseGateway entry vectorTagsExpressionMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) CircRNA Mini Vector
Plasmid#60648PurposeExpression plasmid for expressing circular RNAs of a desired sequenceDepositorInsertZKSCAN1 (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-mtEFTu
Plasmid#30535DepositorInsertMature bovine mitochondrial elongation factor Tu
UseTagsHisExpressionBacterialMutationMature form amino acids 44-452. Mito import seque…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQE-9-RTXMCR-TLY-X2A-C
Plasmid#225971PurposeRepeats-In-Toxin (RTX) protein consensus sequence GGAGADTLY repeated nine times with C-terminal Capping DomainDepositorInsertRTMCR-TLY-X2A-C
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE-9-RTXMCR-TLY-X2H-C
Plasmid#225972PurposeRepeats-In-Toxin (RTX) protein consensus sequence GGAGHDTLY repeated nine times with C-terminal Capping DomainDepositorInsertRTXMCR-TLY-X2H-C
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE-9-RTXMCR-TLY-X2S-C
Plasmid#225973PurposeRepeats-In-Toxin (RTX) protein consensus sequence GGAGSDTLY repeated nine times with C-terminal Capping DomainDepositorInsertRTXMCR-TLY-X2S-C
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE-9-RTXMCR-TLY-X2N-C
Plasmid#225974PurposeRepeats-In-Toxin (RTX) protein consensus sequence GGAGNDTLY repeated nine times with C-terminal Capping DomainDepositorInsertRTXMCR-TLY-X2N-C
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE-9-RTXMCR-TLY-X2D-C
Plasmid#225975PurposeRepeats-In-Toxin (RTX) protein consensus sequence GGAGDDTLY repeated nine times with C-terminal Capping DomainDepositorInsertRTXMCR-TLY-X2D-C
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE-9-RTXMCR-TLY-X2E-C
Plasmid#225976PurposeRepeats-In-Toxin (RTX) protein consensus sequence GGAGEDTLY repeated nine times with C-terminal Capping DomainDepositorInsertRTXMCR-TLY-X2E-C
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
HS_NAM-B1
Plasmid#154064PurposeLevel M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosTDepositorInsertsHygromycin phosphotransferase
Cre recombinase
LoxP-flanked GUS and TtNAM-B1
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe TtNAM-B1 gene sequence was domesticated to re…PromoterHvHSP17, OsAct, and ZmUbiAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
C40m
Plasmid#120973PurposeMoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertBlank (Random 50bp sequence)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
eft-3p:act-5(ptc):tbb2 3' UTR
Plasmid#202331PurposeThe ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.DepositorInsertseft-3 promoter
act-5(ptc)
tbb-2 3' UTR
UseTagsbase 238 C -> A mutation causing a PTC at codo…ExpressionWormMutationPromoterThis is a promoter sequence for C. elegans eft-3,…Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPK-352
Plasmid#157922PurposepcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNRDepositorInsertstPcyA
IRES
HO1
FD
FNR
UseSynthetic Biology; OptogeneticsTagsMitochondrial Targeting Sequence, Mitochondrial T…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
p210 pCMV-CREM
Plasmid#8395PurposeCre with chimeric/β-globin intron within the Cre coding sequenceDepositorInsertCREM
UseCre/LoxTagsExpressionMammalianMutationmodified CrePromoterCMVAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual
Plasmid#216377PurposeBaculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)DepositorInsertLDL receptor related protein 6 (LRP6 Human)
UseBaculovirusTags9xHis and HA signal sequence-FLAG-3C cleavage sit…ExpressionInsectMutationPromoterPolyhedrin (LRP6)/p10 (MESD)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only