Skip to main content
Addgene
Showing: 781 - 800 of 3083 results

  3. pcDNA3.1(+)-FluoSTEP-JNKAR

    Plasmid
    #181857
    Purpose
    Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-JNKAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181857-plasmid-map-sequence-id-363201

    Enlarge

  5. pcDNA3-Lyso-AktAR2

    Plasmid
    #64933
    Purpose
    Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.
    Depositor
    Jin Zhang
    Article
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Insert
    LAMP1-AktAR2 (LAMP1 Synthetic, Budding Yeast, Human)
    Use
    Tags
    Cerulean3, Full-length LAMP1, and cpVenus[E172]
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64933-plasmid-map-sequence-id-109712

    Enlarge

  6. pcDNA3-Akt-STOPS

    Plasmid
    #175006
    Purpose
    Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.
    Depositor
    Jin Zhang
    Article
    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:
    Insert
    mCherry-Akt-STOPS
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    175006-plasmid-map-sequence-id-344970

    Enlarge

  8. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid
    #181858
    Purpose
    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-EKAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181858-plasmid-map-sequence-id-363410

    Enlarge

  15. pcDNA3-HA-14-3-3 epsilon

    Plasmid
    #13273
    Depositor
    Michael Yaffe
    Insert
    14-3-3 epsilon (YWHAE Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 781 - 800 of 3083 results