Addgene

We narrowed to 4,062 results for: p2a

Showing: 1171 - 1200 of 4062 results

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pAAV-hPhyB621(Y276H)-P2A-m1V

Plasmid

#197377

Purpose

The AAV vector for PhyB621(Y276H) and m1V

Depositor

Michiyuki Matsuda

Insert

Phytochrome B Y276H(1-621 aa)

Use

AAV

Tags

P2A-m1V

Expression

Mutation

Human codon optimized PhyB (1-621 aa), Y276H muta…

Promoter

CMV

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCSIIbleo-NIR-GECO2-P2A-EGFP

Plasmid

#198063

Purpose

The lentiviral vector for NIR-GECO2 and EGFP

Depositor

Michiyuki Matsuda

Insert

NIR-GECO2

Use

Lentiviral

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCSIIbleo-NIR-GECO1-P2A-EGFP

Plasmid

#198065

Purpose

The lentiviral vector for NIR-GECO1 and EGFP

Depositor

Michiyuki Matsuda

Insert

NIR-GECO1

Use

Lentiviral

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb713-P2A-GFP

Plasmid

#197172

Purpose

Expresses the protein of mRhubarb713-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubard713-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.8-P2A-GFP

Plasmid

#197171

Purpose

Expresses the protein of BDFP1.8-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.8-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP2-P2A-GFP

Plasmid

#197155

Purpose

Expresses the protein of miRFP2-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP2-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-CSRNP2-P2A-mCherry

Plasmid

#203867

Purpose

Tet-inducible lentiviral plasmid expressing CSRNP2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

CSRNP2 (CSRNP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-PBX4-P2A-mCherry

Plasmid

#203864

Purpose

Tet-inducible lentiviral plasmid expressing PBX4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

PBX4 (PBX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-CHMP2A-myc, L216D/L219D

Plasmid

#180645

Purpose

Mammalian expression of CHMP2A with L216D/L219D mutations. Has C-terminal Myc tag. Internal ID:WISP20-13.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP2A (CHMP2A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

L216D, L219D

Promoter

pCAG

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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lentiTet-CSK-P2A-mCherry BlastR

Plasmid

#186742

Purpose

Dox inducible CSK-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

CSK (CSK Human)

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP670-2-P2A-GFP

Plasmid

#197170

Purpose

Expresses the protein of miRFP670-2-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP670-2-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP670-P2A-GFP

Plasmid

#197169

Purpose

Expresses the protein of iRFP670-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP670-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mCardinal-P2A-GFP

Plasmid

#197167

Purpose

Expresses the protein of mCardinal-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mCardinal-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP713-P2A-GFP

Plasmid

#197166

Purpose

Expresses the protein of iRFP713-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP713-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-E2-Crimson-P2A-GFP

Plasmid

#197162

Purpose

Expresses the protein of E2-Crimson-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

E2-Crimson-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-emiRFP703-P2A-GFP

Plasmid

#197158

Purpose

Expresses the protein of emiRFP703-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP703-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-IFP2-P2A-GFP

Plasmid

#197161

Purpose

Expresses the protein of IFP2-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

IFP2-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb720-P2A-GFP

Plasmid

#197159

Purpose

Expresses the protein of mRhubarb720-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb720-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.6-P2A-GFP

Plasmid

#197154

Purpose

Expresses the protein of BDFP1.6-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.6-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb719-P2A-GFP

Plasmid

#197152

Purpose

Expresses the protein of mRhubarb719-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb719-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV/CBA::EGFP-P2A-myc-C3

Plasmid

#129478

Purpose

AAV2 transfer plasmid for myc-tagged C3 with EGFP under control of the CBA promoter

Depositor

Robert Gross

Insert

Myc-tagged (N-terminus) exoenzyme C3 from C. botulinum, P2A, EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV/CBA::EGFP-P2A-tC3

Plasmid

#129476

Purpose

AAV2 transfer plasmid for truncated C3 with EGFP under control of the CBA promoter

Depositor

Robert Gross

Insert

truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-RCaMP1h

Plasmid

#191467

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

RCaMP1h

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmPop2a-D53AE55A-dsRNAres-V5His6_N

Plasmid

#146977

Purpose

Insect Expression of DmPop2a-D53AE55A-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmPop2a-D53AE55A-dsRNAres (Pop2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPop2a-dsRNAres-V5His6_K

Plasmid

#146719

Purpose

Insect Expression of DmPop2a-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmPop2a-dsRNAres (Pop2 Fly)

Use

Tags

Expression

Insect

Mutation

ORF extracted from CG5684-Pa; NM_140281.2, with o…

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPop2a-D53AE55A-V5His6_G

Plasmid

#146370

Purpose

Insect Expression of DmPop2a-D53AE55A

Depositor

Elisa Izaurralde

Insert

DmPop2a-D53AE55A (Pop2 Fly)

Use

Tags

Expression

Insect

Mutation

one non silent variation I164F compared to the se…

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-EGFP

Plasmid

#191458

Purpose

Compare the intensity of EGFP to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

EGFP

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y

Plasmid

#191459

Purpose

Compare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mTurquoise2_T203Y

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-mCherry

Plasmid

#191466

Purpose

Compare the intensity of mCherry to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mCherry

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-GCaMP3

Plasmid

#191460

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

GCaMP3

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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