We narrowed to 9,186 results for: sacs

1. pJS506

   Plasmid

   #200712

   Purpose

   Promoter region from ATPase PMA1 as Pro5U promoter module for heterologous expression of plasma membrane localized transporters in yeast

   Depositor

   Jens Boch

   Article

   Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

   Insert

   PMA1 promoter (PMA1 Budding Yeast)
   

   Use

   Synthetic Biology; Modular cloning

   Tags

   Expression

   Mutation

   domesticated for MoClo cloning (removal of intern…

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCfB9339 (pgRNA_VII-1_NatMX)

   Plasmid

   #161591

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VII-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCfB9340 (pgRNA_VIII-1_NatMX)

   Plasmid

   #161592

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VIII-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCfB9342 (pgRNA_XIII-1_NatMX)

   Plasmid

   #161594

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB9344 (pgRNA_XVI-1_NatMX)

   Plasmid

   #161596

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KBB438

   Plasmid

   #185092

   Purpose

   GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

   Depositor

   Ken Belanger

   Article

   Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

   Insert

   RFA1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Rfa1 with amino acids 338-343 deleted

   Promoter

   GAL1

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KBB372

   Plasmid

   #185084

   Purpose

   GAL::Rfa1[330-621]-GFP under control of GAL1 promoter

   Depositor

   Ken Belanger

   Article

   Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

   Insert

   RFA1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Rfa1 amino acids 330-621

   Promoter

   GAL1

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMM0649

   Plasmid

   #129010

   Purpose

   Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMM0655

   Plasmid

   #129016

   Purpose

   Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMM0654

    Plasmid

    #129015

    Purpose

    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMM0653

    Plasmid

    #129014

    Purpose

    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMM0650

    Plasmid

    #129011

    Purpose

    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMM0648

    Plasmid

    #129009

    Purpose

    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMM0651

    Plasmid

    #129012

    Purpose

    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTJK502

    Plasmid

    #121457

    Purpose

    CEN HIS3 CNB1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Promoter

    Endogenous genomic promoter region

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTJK513

    Plasmid

    #121470

    Purpose

    CEN LEU2 CNB1mutEF1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF1 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Changed aspartic acid 34 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTJK511

    Plasmid

    #121468

    Purpose

    2µm HIS3 CNB1mutEF4

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF4 (CNB1 Budding Yeast)
    

    Use

    Yeast high copy 2 micron vector

    Tags

    Expression

    Mutation

    Changed aspartic acid 144 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTJK510

    Plasmid

    #121465

    Purpose

    2µm HIS3 CNB1mutEF3

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF3 (CNB1 Budding Yeast)
    

    Use

    Yeast high copy 2 micron vector

    Tags

    Expression

    Mutation

    Changed aspartic acid 103 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTJK509

    Plasmid

    #121464

    Purpose

    2µm HIS3 CNB1mutEF2

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF2 (CNB1 Budding Yeast)
    

    Use

    Yeast high copy 2 micron vector

    Tags

    Expression

    Mutation

    Changed aspartic acid 66 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTJK508

    Plasmid

    #121463

    Purpose

    2µm HIS3 CNB1mutEF1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF1 (CNB1 Budding Yeast)
    

    Use

    Yeast high copy 2 micron vector

    Tags

    Expression

    Mutation

    Changed aspartic acid 34 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTJK516

    Plasmid

    #121473

    Purpose

    CEN LEU2 CNB1mutEF4

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF4 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Changed aspartic acid 144 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTJK506

    Plasmid

    #121461

    Purpose

    CEN HIS3 CNB1mutEF4

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF4 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Changed aspartic acid 144 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTJK505

    Plasmid

    #121460

    Purpose

    CEN HIS3 CNB1mutEF3

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF2 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Changed aspartic acid 103 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTJK504

    Plasmid

    #121459

    Purpose

    CEN HIS3 CNB1mutEF2

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF2 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Changed aspartic acid 66 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTJK503

    Plasmid

    #121458

    Purpose

    CEN HIS3 CNB1mutEF1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF1 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Changed aspartic acid 34 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTJK518

    Plasmid

    #121475

    Purpose

    CEN TRP1 CNA1ΔAID

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNA1deltaAID
    

    Use

    Yeast cen vector

    Tags

    Expression

    Mutation

    Truncated after amino acid 508

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTJK519

    Plasmid

    #121476

    Purpose

    CEN TRP1 CNA1ΔCBD

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNA1deltaCBD
    

    Use

    Yeast cen vector

    Tags

    Expression

    Mutation

    Truncated after amino acid 453

    Promoter

    Endogenous genomic promoter region

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTJK507

    Plasmid

    #121462

    Purpose

    2µm HIS3 CNB1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1 (CNB1 Budding Yeast)
    

    Use

    Yeast high copy 2 micron vector

    Tags

    Expression

    Mutation

    Promoter

    Endogenous genomic promoter region

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRPL28 SpHIS5 (pNTI663)

    Plasmid

    #115433

    Purpose

    Expresses wild-type RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRPL28(Q38P) SpHIS5 (pNTI664)

    Plasmid

    #115434

    Purpose

    Expresses Q38P RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q38P; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge