We narrowed to 3,025 results for: ER

1. pFSynW Syt1 D303G IRES mRuby3

   Plasmid

   #170640

   Purpose

   Expression of syt1 in cultured neurons by lentiviral transduction

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D303G

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFSynW Syt1 D365E IRES mRuby3

   Plasmid

   #170641

   Purpose

   Expression of syt1 in cultured neurons by lentiviral transduction

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D365E

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX Syt1 C2AB (96-421) D303G

   Plasmid

   #170646

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D303G

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX Syt1 C2AB (96-421) D365E

   Plasmid

   #170649

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D365E

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX Syt1 C2AB (96-421) I367T

   Plasmid

   #170652

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I367T

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pU6-cGAS g2 Cas9-T2A-mCherry

   Plasmid

   #164253

   Purpose

   Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgcGAS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. EcMBP165-cpGFP.PPYF.T203V.pRSET

   Plasmid

   #33373

   Purpose

   a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

   Depositor

   Loren Looger

   Article

   Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

   Insert

   Improved MBP based Maltose Biosensor
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from GCaMP2 inserted between AA1…

   Promoter

   T7

   Available since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-INU-FLAG-FAM134Bs

   Plasmid

   #128261

   Purpose

   Expresses FAM134Bs in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   FAM134Bs (RETREG1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEM705 Gso (Ser)

   Plasmid

   #109353

   Purpose

   GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gso chimera
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cysteine at position 391 to Serine (Pertu…

   Promoter

   CAG

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFSW Syt1 WT IRES GFP

    Plasmid

    #133816

    Purpose

    Encodes full-length wild type synaptotagmin 1 for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cenp-B INCENP GFP

    Plasmid

    #45238

    Purpose

    Target INCENP to centromeres via full length CENP-B

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B, INCENP-Δcen, GFP
    

    Use

    Tags

    GFP and full length CENP-B

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-Diaphanous-GFP

    Plasmid

    #24287

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Diaphanous (dia Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

13. EGFP-Miro1v4

    Plasmid

    #174111

    Purpose

    Expresses the peroxisomal variant (splice variant 4) of Miro with an N-terminal EGFP.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Miro1 (var4, peroxisomal splice variant)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEM705 Gso (Cys)

    Plasmid

    #109375

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRN3P_TIR1

    Plasmid

    #119766

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    TIR1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-cGAS g3 Cas9-T2A-mCherry

    Plasmid

    #164254

    Purpose

    Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgcGAS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRN3P_Kdm5b_MUT

    Plasmid

    #119767

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    Kdm5b MUT
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEM705 Gsi (Cys)

    Plasmid

    #109373

    Purpose

    GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsi chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LacI INCENP

    Plasmid

    #45236

    Purpose

    Target INCENP to lac operator sequence

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    LacI, INCENP-Δcen, mCherry (lacI )
    

    Use

    Tags

    LacI (lac repressor protein) and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    t7

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. VPS13D^Halo

    Plasmid

    #174108

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    With an internal Halo tag in aminoacid position 1…

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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