We narrowed to 1,068 results for: FIB

1. pSAM2_mCherry_Isl1

   Plasmid

   #72696

   Purpose

   Doxycyline regulated expression of Isl1 in mammalian cells

   Depositor

   Timothy Kamp

   Article

   Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

   Insert

   Islet1 (Isl1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV-Doxycycline

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PA-DAD-wt

   Plasmid

   #53095

   Purpose

   Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.

   Depositor

   Klaus Hahn, Ronen Zaidel-Bar

   Article

   Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

   Insert

   LOV(wt)-6aa-DAD
   

   Tags

   6x His and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   L47F and C49S in Venus

   Available Since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKrox24(MapERK)d1EGFP (KroxDs)

   Plasmid

   #214912

   Purpose

   for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   d1EGFP under the MapERK promoter
   

   Expression

   Mammalian

   Promoter

   MapkERK promoter

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTWIN1-His6-Ssp-Httex1-23Q

   Plasmid

   #84349

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTWIN1-His6-Ssp-Httex1-7Q

   Plasmid

   #84347

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 7Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Tags

   N-terminal His6-tagged intein Ssp

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-Elf5 gRNA

   Plasmid

   #128836

   Purpose

   gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   mElf5 gRNA (Elf5 Mouse)
   

   Use

   CRISPR

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-syn-FLEX-jYCaMP1

   Plasmid

   #135422

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Insert

   jYCaMP1
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-OptoFGFR1-Y766F

   Plasmid

   #59777

   Purpose

   Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

   Depositor

   Won Do Heo

   Article

   Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

   Insert

   OptoFGFR1-Y766F (FGFR1 Human, Mustard Weed)
   

   Tags

   mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

   Plasmid

   #128835

   Purpose

   Knock-in of TagBFP into mouse Esrrb locus

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   tagBFP into Esrrb locus (Esrrb Mouse)
   

   Use

   Mouse Targeting

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTWIN1-His6-Ssp-Httex1-29Q

    Plasmid

    #84350

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 29Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP Kind2 S159/181/666A

    Plasmid

    #105310

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666A

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTWIN1-His6-Ssp-Httex1-37Q

    Plasmid

    #84351

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTWIN1-His6-Ssp-Httex1-15Q

    Plasmid

    #84348

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Esrrb gRNA

    Plasmid

    #128841

    Purpose

    gRNA for targeting mouse Esrrb locus using CRISPR-cas technique

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Esrrb gRNA (Esrrb Mouse)
    

    Use

    CRISPR

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRCA377 - pBA904 Puro-T2A-GFP CD55 CRISPRa guide 1 (pRCA360 backbone)

    Plasmid

    #238168

    Purpose

    Lentiviral CRISPR guide vector expressing CD55 targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    CD55 sgRNA CRISPRa (CD55 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRCA810 - pBA904 Puro-T2A-GFP HES7 g2 CRISPRa guide (pRCA360 backbone) 674

    Plasmid

    #238177

    Purpose

    Lentiviral CRISPR guide vector expressing a HES7 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    HES7 sgRNA CRISPRa (HES7 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE

    Plasmid

    #229544

    Purpose

    Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein

    Depositor

    Domitilla Del Vecchio

    Article

    Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

    Insert

    Oct4(co)-mNeonGreen (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Oct4-mNeonGreen

    Expression

    Mammalian

    Mutation

    codon-optimized OCT4

    Promoter

    TRE12

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. ATX3-D1

    Plasmid

    #184246

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
    

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Premature stop codon N263X

    Promoter

    T7 promoter

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep

    Plasmid

    #180314

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1-i5

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 )
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    SEPT9_i1-i5

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_SEPT9_i1 S22A S23A-msfGFP

    Plasmid

    #180338

    Purpose

    mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 S22A S23A

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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