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Showing: 781 - 800 of 1019 results

  1. pTight-9-124-BclxL

    Plasmid
    #60857
    Purpose
    2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter
    Depositor
    Andrew Yoo
    Article
    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1
    Insert
    miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pTight
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60857-plasmid-map-sequence-id-195309

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  2. pET-28a(+)-FGF2-G3

    Plasmid
    #135521
    Purpose
    Engineered form of fibroblast growth factor 2 with improved thermostability
    Depositor
    Paul Burridge
    Article
    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221
    Insert
    fibroblast growth factor 2 (FGF2 Human)
    Use
    Tags
    6x His tag and Agg Thrombin Tag
    Expression
    Bacterial
    Mutation
    Codon optimized for E. Coli production
    Promoter
    T7 Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135521-plasmid-map-sequence-id-273044

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  11. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

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  12. pPB-tetpA(hBCL2-miR9/9*-124)-iRPT

    Plasmid
    #154881
    Purpose
    Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.
    Depositor
    Hideyuki Okano
    Article
    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001
    Inserts
    iRFP713
    Puromycin resistance gene
    rtTAM2
    BCL2 (BCL2 Human)
    miR9/9*-124
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    154881-plasmid-map-sequence-id-313227

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  13. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

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  15. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184248-plasmid-map-sequence-id-370322

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  18. pAAV-syn-FLEX-axon-jYCaMP1s

    Plasmid
    #135421
    Purpose
    Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant
    Depositor
    Kaspar Podgorski
    Article
    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s
    Has Service
    AAV1
    Insert
    jYCaMP1s
    Use
    AAV
    Tags
    GAP43 axon targeting sequence
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Jan. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    135421-plasmid-map-sequence-id-268929

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Showing: 781 - 800 of 1019 results