Addgene

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MEIS1B-pSP65

Plasmid

#21019

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1B (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Expression

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
HOXA9 (murine) FLAG pET

Plasmid

#8494

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
px330-UFSP2 sgRNA1

Plasmid

#134638

Purpose

contains sgRNA targeting human UFSP2 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFSP2 sgRNA1 (UFSP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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HOXB13 (human) HIS-tag pET

Plasmid

#8543

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

HOXB13 (HOXB13 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Western and by transcription/tra…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB13 (human) Myc-tag-GST

Plasmid

#8683

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

HOXB13 (HOXB13 Human)

Use

Tags

GST and Myc

Expression

Bacterial

Mutation

makes protein by western

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
sh-Myocardin

Plasmid

#100768

Purpose

expression of shRNA targeting MYOCD

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

sh-Myocardin

Use

RNAi

Tags

Expression

Mutation

Promoter

H1

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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HOXC13(human) GSTfusion-pET

Plasmid

#8680

Depositor

Corey Largman

Article

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB13 (human) Myc-tag-Bluescript

Plasmid

#8684

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

HOXB13 (HOXB13 Human)

Use

Tags

Myc

Expression

Bacterial

Mutation

Myc tag on N-terminal

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXD13 (chicken) HIS-tag pET

Plasmid

#8555

Depositor

Corey Largman

Article

Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206

Insert

HOXD13 (HOXD13 )

Use

Tags

His and T7

Expression

Bacterial

Mutation

contains 10 nt 5' to ATG start; makes prote…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
mCherry-FKBP-PIP4K2A-Kina

Plasmid

#202745

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108) : [GGSA]4GG :PIP4K2A (33-406) (PIP4K2A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRD445

Plasmid

#168775

Purpose

Expression of mEos3.2 in Mycobacterium

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mEos3.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRWJB006

Plasmid

#160113

Purpose

Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

35S

Available since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRWJB004

Plasmid

#160112

Purpose

Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

UBQ10

Available since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Puro-TTL-TTF-1-HDD

Plasmid

#74989

Purpose

Human TTF-1 (NKX2-1) cDNA with the homeodomain deleted

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 HDD mutant (NKX2-1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Homeodomain

Promoter

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Alx3

Plasmid

#80382

Purpose

mammalian expression of rat Alx3

Depositor

Mario Vallejo

Article

Perez-Villamil et al J Biol Chem. 2004 Sep 3;279(36):38062-71

Insert

Alx3 (Alx3 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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Zebrafish lim5 (lhx5)

Plasmid

#74141

Purpose

Basic cDNA clone for zebrafish lim5 (lhx5). Cloned in BS KS+.

Depositor

Igor Dawid

Article

Toyama et al Dev Biol. 1995 Aug;170(2):583-93.

Insert

lhx5 (lhx5 Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only
HOXB13 (human) Myc-tag-pSP72

Plasmid

#8682

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

HOXB13 (HOXB13 Human)

Use

Tnt in vitro

Tags

Myc

Expression

Bacterial

Mutation

new NsiI site at ATG start

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-TFE3

Plasmid

#38120

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFE3 (TFE3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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B1-INTEGRIN WT

Plasmid

#195215

Purpose

Mammalian expression of mCherry tagged B1 Integrin. Wild-type.

Depositor

Valerie Weaver, David Bryant

Article

Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

Insert

ITGB1 (ITGB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-PSDΔ1,2-FRBx3

Plasmid

#225827

Purpose

A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

Depositor

Haruo Kasai

Article

Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

Insert

PSDΔ1,2-FRBx3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge