We narrowed to 29,072 results for: PLE;

1. pLenti-hSyn-PROGERIN

   Plasmid

   #140189

   Purpose

   Overexpression of Progerin with a GFP fusion protein, under human synapsin promotor

   Depositor

   Lorenz Studer

   Article

   Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

   Insert

   Progerin (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. plex_307-ccdB-iRFP713

   Plasmid

   #201140

   Purpose

   pLEX_307 plasmid with iRFP713 marker

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. plex_307-ccdB-EYFP

   Plasmid

   #201146

   Purpose

   pLEX_307 plasmid with EYFP marker

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEGFP-Y705F-STAT3

   Plasmid

   #71445

   Purpose

   Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

   Depositor

   George Stark

   Article

   Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   Y705F

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

5. PB-TO-hNIL BSD-mApple

   Plasmid

   #182313

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   hNGN2 (NEUROG2 Human)
   hISL1 (ISL1 Human)
   hLHX3 (LHX3 Human)
   bsd-T2A-mycNLS-mApple
   

   Use

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti Dest BlastR R1-R2

   Plasmid

   #84574

   Purpose

   Gateway destination vector expressing Blasticidin resistance under a PGK promoter

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Type

   Empty backbone

   Use

   Lentiviral; Gateway

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-basP:fLuc-TK:rLuc

   Plasmid

   #138369

   Purpose

   Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   sequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRUPATH Triple Gagust

   Plasmid

   #196054

   Purpose

   Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaGustducin-RLuc8 (GNAT3 Human)
   GGamma1-GFP2 (GNGT1 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_rtTA-P2A-BFP_HygroR

   Plasmid

   #186738

   Purpose

   rtTA-P2A-BFP expression construct cloned into lentiviral backbone

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   rtTA
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.2_mCherry_ mito_roGFP2-Orp1

    Plasmid

    #155042

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    mitochondrial targeting sequence and roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCas9-T2A-BFP

    Plasmid

    #78547

    Purpose

    Cas9 protein with BFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. plentiCMV-sp-HPV-TM(ICAM)

    Plasmid

    #196510

    Purpose

    Express HPV antigen epitope with ICAM transmembrane domain

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    a HPV epitope with CD8a stalk linker and ICAM TM domain
    

    Use

    Lentiviral

    Tags

    3xflag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. plenti hSyn PKM1

    Plasmid

    #220920

    Purpose

    Neuronal specific expression of PKM1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PKM1 (PKM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX307-DPP4-puro

    Plasmid

    #158451

    Purpose

    Constitutive lentiviral expression of human DPP4.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    DPP4 (DPP4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. plenti-FLAG-CAMK2D

    Plasmid

    #221699

    Purpose

    Lentiviral expression of FLAG-tagged human CAMK2D in mammalian cells

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_CMV:Flag-NLuc_SV40:PuroR

    Plasmid

    #183045

    Purpose

    Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    NanoLuciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-FoxO1-Clover

    Plasmid

    #67759

    Purpose

    Fluorescent fusion protein for tracking Akt signaling

    Depositor

    Peter Rotwein

    Article

    Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

    Insert

    Fox01 (Foxo1 Mouse)
    

    Use

    Lentiviral

    Tags

    Clover

    Expression

    Mutation

    Changed Serine 209 to Alanine, changed Histidine …

    Promoter

    EF1a

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSimple-miR302/367

    Plasmid

    #98748

    Purpose

    Episomal reprogramming

    Depositor

    Sara Howden, James Thomson

    Article

    Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

    Insert

    miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-EB1-EGFP

    Plasmid

    #118084

    Purpose

    Expresses EB1-EGFP in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    human EB1 (MAPRE1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6/V5-DEST-FASCIN

    Plasmid

    #31207

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    FASCIN (FSCN1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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