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pCB116

Plasmid

#53365

Purpose

To express Cup9p‐FLAG from the PGAL1 promoter

Depositor

Alexander Varshavsky

Article

Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

Insert

Cup9 (CUP9 Budding Yeast)

Tags

Flag

Expression

Yeast

Promoter

GAL1

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pCB210

Plasmid

#31722

Purpose

To express Cup9p‐FLAG from the PCUP9 promoter

Depositor

Alexander Varshavsky

Article

Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

Insert

CUP9 (CUP9 Budding Yeast)

Tags

Cup9 promoter and FLAG

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pBTK1211 (pDS-BiFC7)

Plasmid

#251992

Purpose

Double-stranded RNA biomolecular fluorescence complementation sensor for bacteria, induced by arabinose.

Depositor

Jeffrey Barrick

Article

Navarro-Escalante et al bioRxiv 2026.01.18.700149

Insert

Split mVenus (YFP) fused to mutant B2 dsRNA-binding domains (RBDs)

Use

Biosensor

Expression

Bacterial

Promoter

PBAD

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pBTK1210 (pDS-BiFC6)

Plasmid

#251991

Purpose

Double-stranded RNA biomolecular fluorescence complementation sensor for bacteria, induced by vanillic acid.

Depositor

Jeffrey Barrick

Article

Navarro-Escalante et al bioRxiv 2026.01.18.700149

Insert

Split mVenus (YFP) fused to mutant B2 dsRNA-binding domains (RBDs)

Use

Biosensor

Expression

Bacterial

Promoter

Pvan

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pEM7065

Plasmid

#214003

Purpose

PrpsM driven expression of ssrA-tagged mCerulean (LVA)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCerulean-LVA

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7064

Plasmid

#214002

Purpose

PrpsM driven expression of ssrA-tagged mNeonGreen (LVA)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen-LVA

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM9177

Plasmid

#213994

Purpose

PrpsM driven expression of SSAGSAAGS-mGFPmut2

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

SSAGSAAGS-mGFPmut2

Tags

SSAGSAAGS

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7071

Plasmid

#214009

Purpose

PrpsM driven expression of ssrA-tagged mScarlet-I (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mScarlet-I-ASV

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pNB687

Plasmid

#60119

Purpose

Yellow beetle luciferase

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

YeLuc

Use

Unspecified

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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UPR pBAM Ura3

Plasmid

#45355

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

DHFR Ub Mek Ura3

Expression

Yeast

Promoter

pCUP1

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-(empty)-TAG

Plasmid

#138209

Purpose

To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

Depositor

Neal Devaraj

Article

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Type

Empty backbone

Use

T7 transcription

Tags

TAG sequence

Expression

Mammalian

Promoter

CMV Promoter, T7 Promoter

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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MAC3-tag-N

Plasmid

#185482

Purpose

Gateway compatible destination vectors containing StrepIII/HA/UltralD

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Type

Empty backbone

Tags

StrepIII-HA-UltraID

Expression

Bacterial and Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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MAC3-tag-C

Plasmid

#185481

Purpose

Gateway compatible destination vectors containing StrepIII/HA/UltralD

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Type

Empty backbone

Tags

StrepIII-HA-UltraID

Expression

Bacterial and Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL Luciferase

Plasmid

#120798

Purpose

For production of lentivirus to express luciferase, primarily for in vivo bioluminescence tracking experiments.

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

hPGK

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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MAC3-N-GFP

Plasmid

#222602

Purpose

Expression of MAC3-tagged GFP (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pYTK-DN2

Plasmid

#180283

Purpose

Spacer cassette (TU3)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConL2 - Spacer - ConRE

Expression

Bacterial

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK-DN3

Plasmid

#180284

Purpose

Spacer cassette (TU2)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConL1 - Spacer - ConR2

Expression

Bacterial

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG UBR1 SBX

Plasmid

#24506

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 (UBR1 Budding Yeast)

Tags

FLAG

Expression

Yeast

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+/ER-2mutAEQ

Plasmid

#45541

Depositor

Javier Alvarez-Martin

Article

de la Fuente et al Biochem J. 2012 Aug 1;445(3):371-6. doi:

Insert

ER-2mutAEQ

Tags

ER targeted

Expression

Mammalian

Mutation

Asp119Ala and Asn28Leu

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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pGL3-PMT-miR-34a

Plasmid

#25799

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-34a promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only