We narrowed to 13,942 results for: cas9

1. ADEPT-pCas9

   Plasmid

   #238032

   Purpose

   Encodes Cas9 (ssrA degradation-tagged) under a tetracycline promoter (pTet) on a low-copy replication origin (SC101) as part of the ADEPT system.

   Depositor

   Lingchong You

   Article

   Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

   Insert

   spCas9
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Promoter

   tet

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQdCas9-sggfp

   Plasmid

   #236184

   Purpose

   The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
   

   Promoter

   Quorum sensing promoter

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEVA47_dCas9-GFP_NOT_iC

   Plasmid

   #231418

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9-tdTomato

   Plasmid

   #232631

   Purpose

   catalytically inactive dCas9 for localization; labeled with tdTomato

   Depositor

   Karsten Rippe

   Insert

   dCas9-tdTomato
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA47_dCas9-GFP_AAN_4

   Plasmid

   #231415

   Purpose

   This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   sgRNA-X
   sgRNA-Y
   

   Use

   Synthetic Biology

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEVA47_dCas9-GFP_NOT_iA

   Plasmid

   #231417

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEVA47_dCas9-GFP_YES_i2

   Plasmid

   #231419

   Purpose

   This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   sgRNA-Y
   

   Use

   Synthetic Biology

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEVA47_dCas9-GFP_NOT_i1

   Plasmid

   #231416

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

   Plasmid

   #222988

   Purpose

   Bacterial expression of iGeoCas9(C2) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-miniCGBE1

    Plasmid

    #198548

    Purpose

    Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-miniCGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-NG-miniCGBE1

    Plasmid

    #198538

    Purpose

    Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-miniCGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-NRRH

    Plasmid

    #198540

    Purpose

    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRRH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-NRCH

    Plasmid

    #198542

    Purpose

    Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. DG SaCas9 GFP V3

    Plasmid

    #226961

    Purpose

    CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. DG SaCas9 puro V3

    Plasmid

    #226960

    Purpose

    CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCas9-FCPF

    Plasmid

    #220131

    Purpose

    Expresses dCas9-FCPF in mammalian cells

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    dCas9-FCPF (cas9 S. pyogenes, Synthetic)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgRNA1_Cas9-hGem_Smc4

    Plasmid

    #217667

    Purpose

    expresses Cas9-hGem and guideRNA for tagging of SMC4

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA1 targeting SMC4 C-terminal (SMC4 Human)
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

    Plasmid

    #222987

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-Cas9-NS-A

    Plasmid

    #207823

    Purpose

    Dual expression of Cas9 and non-specific sgRNA

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    non-specific sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Promoter

    EF-1a; U6

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-Cas9-NS-B

    Plasmid

    #207824

    Purpose

    Dual expression of Cas9 and non-specific sgRNA

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    non-specific sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Promoter

    EF-1a; U6

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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