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We narrowed to 2,389 results for: dap

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Showing: 781 - 800 of 2389 results

  1. p35u4

    Plasmid
    #174786
    Purpose
    Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system
    Depositor
    Katherine Berry
    Article
    Wang et al RNA. 2021 Apr;27(4):513-526. doi: 10.126
    Insert
    MS2 coat protein
    Tags
    λCI
    Expression
    Bacterial
    Mutation
    V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…
    Promoter
    Synthetic Constitutive Promoter
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174786-plasmid-map-sequence-id-361842

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  2. pCR4-Dact1(1250-1601)

    Plasmid
    #52046
    Purpose
    To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.
    Depositor
    Ben Cheyette
    Article
    Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.
    Insert
    Dact1(1250-1601) (Dact1 Mouse)
    Expression
    Bacterial
    Available Since
    April 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pGEMHE-MmCENPT-4NS-GFP

    Plasmid
    #237431
    Purpose
    Expresses Mus musculus CENP-T with 4 rat-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with 4 rat-specific residues in HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    432 T to A; 463 I to T, 470 L to E, 485 Q to L
    Promoter
    T7
    Available Since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    237431-plasmid-map-sequence-id-474223

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  6. pGEMHE-MmCENPT-RnHFD-4NS-GFP

    Plasmid
    #237432
    Purpose
    Expresses Mus musculus CENP-T with rat HFD and 4 mouse-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Rattus norvegicus HFD and 4 mouse-specific residues in HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    432 A to T; 463 T to I, 470 E to L, 485 L to Q in…
    Promoter
    T7
    Available Since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237432-plasmid-map-sequence-id-474252

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  8. hsp70: mKate2 dynactin1-811

    Plasmid
    #105970
    Purpose
    heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function
    Depositor
    Caren Norden
    Article
    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10
    Insert
    dynactin1-811 (DCTN1 Human)
    Tags
    mKate2
    Expression
    Bacterial
    Mutation
    contains only amino acids 1-811
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    105970-plasmid-map-sequence-id-203781

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  9. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Article
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Available Since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169375-plasmid-map-sequence-id-333771

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  10. GB_PVX (GB1464)

    Plasmid
    #160612
    Purpose
    PVX vector adapted to GB
    Depositor
    Diego Orzaez
    Insert
    PVX vector adapted to GB
    Use
    Synthetic Biology
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160612-plasmid-map-sequence-id-322027

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  11. pTargetAmp

    Plasmid
    #228552
    Purpose
    Template for PCR amplification for gRNA cloning plasmid by in vivo cloning.
    Depositor
    Jeferson Gross
    Insert
    Ampicillin resistance
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228552-plasmid-map-sequence-id-454861

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  13. pSpec5'

    Plasmid
    #228549
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228549-plasmid-map-sequence-id-454858

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  14. pSpec3'

    Plasmid
    #228548
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228548-plasmid-map-sequence-id-454857

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  16. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228551-plasmid-map-sequence-id-454860

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  17. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

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  18. pCDF-B-pC

    Plasmid
    #213495
    Purpose
    Encodes Node B of the three-node Turing circuit
    Depositor
    Mark Isalan
    Inserts
    cinI (cinI )
    lacI (lacI )
    lambda cI (lambdap50 )
    sfGFP
    Use
    Synthetic Biology
    Tags
    ASV tag and LVA tag
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    213495-plasmid-map-sequence-id-430628

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Showing: 781 - 800 of 2389 results