Addgene

We narrowed to 6,344 results for: sas

Showing: 781 - 800 of 6344 results

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pCS2-foxd4l1.1-A6

Plasmid

#185506

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

FSIENIM to AAAAAAM

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlY282F

Plasmid

#172468

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY282F

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Tyrosine at aa282 to Phenylalanine

Promoter

SP6

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDSARN-vasa-eSpCas9sv40

Plasmid

#173670

Purpose

eSpCas9 expression vector for Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Insert

eSpCas9 with Anopheles vasa promoter and SV40 terminator

Use

CRISPR

Tags

Expression

Insect

Mutation

mutations in Cas9 reducing off-target activity (S…

Promoter

vasa

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlF127P

Plasmid

#174507

Purpose

expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlF127P

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Phenylalanine at aa127 to Proline

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlY55F

Plasmid

#172466

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY55F

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Tyrosine at aa55 to Phenylalanine

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nl

Plasmid

#172463

Purpose

expresses wild-type protein when injected into embryos

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl (wbp2nl.L Frog)

Use

Xenopus expression

Tags

none

Expression

Mutation

none

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-DiB1

Plasmid

#168800

Purpose

Fluorogen activating protein (FAP) DiB1

Depositor

Alexander Mishin

Article

Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

Insert

Fluorogen activating protein DiB1

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-DiB3

Plasmid

#168802

Purpose

Fluorogen activating protein (FAP) DiB3

Depositor

Alexander Mishin

Article

Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

Insert

Fluorogen activating protein DiB3

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pNAM-B1

Plasmid

#154056

Purpose

Level 0 Golden Gate vector, contains domesticated NAM-B1

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

TaNAM-B1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Synonymous mutation at base 3257, in exon 3, to r…

Promoter

N/A

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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LLhE_AW-pHG165 H118R

Plasmid

#90063

Purpose

Constitutive expression of a chimeric LacI:CelR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Sousa et al J Mol Biol. 2016 Feb 22;428(4):671-8. do

Insert

LLhE_AW/H118R

Use

Tags

Expression

Bacterial

Mutation

I48V/Q55A/Q60R/H118R

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ150-lin26

Plasmid

#80357

Purpose

Pmex-5:GFP::H2B::PEST::lin-26 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::lin-26 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCFJ150-atg4.2

Plasmid

#80352

Purpose

Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCFJ150-set6

Plasmid

#80362

Purpose

Pmex-5:GFP::H2B::PEST::set-6 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::set-6 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCFJ150-ets4

Plasmid

#80355

Purpose

Pmex-5:GFP::H2B::PEST::ets-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::ets-4 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-mex3

Plasmid

#80361

Purpose

Pmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::mex-3 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-mbk2

Plasmid

#80360

Purpose

Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS2

Plasmid

#69234

Purpose

U6 driven SpCas9 sgRNA expression for TS2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS2 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS3

Plasmid

#69235

Purpose

U6 driven SpCas9 sgRNA expression for TS3 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS4

Plasmid

#69236

Purpose

U6 driven SpCas9 sgRNA expression for TS4 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

Plasmid

#69221

Purpose

Expresses R1333K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Lysine

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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