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Plasmid#123204PurposeGateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL2 G116A
Plasmid#123087PurposeGateway entry clone encoding human GABARAPL2 G116ADepositorInsertGamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationGlycine 116 to AlaninePromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR E2 HSPter-RPS5Ap-mito
Plasmid#163146PurposePlant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORFDepositorInsertA terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL2 G116
Plasmid#123088PurposeGateway entry clone encoding human GABARAPL2 G116DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationDeleted amino acid 117. Stop codon after G116PromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3A G120
Plasmid#123071PurposeGateway entry clone encoding human MAP1LC3A G120DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseGateway entry vector / entry cloneTagsExpressionMutationDeleted amino acid 121. Stop codon after G120.PromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B G120
Plasmid#123074PurposeGateway entry clone encoding human MAP1LC3B G120DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseGateway entry vector / entry cloneTagsExpressionMutationDeleted amino acids 121-125. Stop codon after G12…PromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL1 G116
Plasmid#123085PurposeGateway entry clone encoding human GABARAPL1 G116DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationDeleted amino acid 117. Stop codon after G116PromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTMiniTrex-mCherry-Shark3
Plasmid#233145PurposeServes as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassetteDepositorInsertsmCherry
Shark3
UseProkaryotic expressionTags3x Ty tagExpressionMutationCodon optimized for Trypanosoma cruzi and changed…PromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL1 no-stop
Plasmid#123210PurposeGateway entry clone encoding human GABARAPL1 lacking stop codon, suitable for C-terminal taggingDepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL2 no-stop
Plasmid#123211PurposeGateway entry clone encoding human GABARAPL2 lacking stop codon, suitable for C-terminal taggingDepositorInsertGamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTMiniTrex-mCherry-Shark1
Plasmid#233143PurposeServes as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassetteDepositorInsertsmCherry
Shark1
UseProkaryotic expressionTags3x Ty tagExpressionMutationCodon optimized for Trypanosoma cruzi and changed…PromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6
Plasmid#48812Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3
Plasmid#48811PurposeThis report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.DepositorInsertnAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsExpressionMammalianMutation*PromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-EcTrp-TGA
Plasmid#228780PurposeaaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codonDepositorInsertsEcTrpRS
EcWtR
UseBaculoviralTagsExpressionMammalianMutationTGA suppressorPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ATG3 WT
Plasmid#129292PurposeGateway entry clone encoding wild-type human ATG3 with a stop codonDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ATG3 K243R
Plasmid#129294PurposeGateway entry clone encoding human ATG3 K243RDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationchanged Lysine 243 to ArgininePromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B G120A no-stop
Plasmid#123206PurposeGateway entry clone encoding human MAP1LC3B G120A lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseGateway entry vector / entry cloneTagsExpressionMutationGlycine 120 to Alanine, stop codon removedPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-PSD95-EGFP-WPRE
Plasmid#133785PurposeCre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cellDepositorInsertPSD95-EGFP (Dlg4 Rat)
UseAAV and Cre/LoxTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas9/pTREX-n
Plasmid#68708PurposeExpresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.DepositorInsertFusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
UseCRISPRTagsFusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …ExpressionMutationC4239T mutation that eliminates a BamHI restricti…PromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-mCherry
Plasmid#50480Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsmCherry fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only