We narrowed to 12,481 results for: cel.2

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pX165-eSpCas9

Plasmid

#140564

Purpose

Expresses eSpCas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

K848A K1003A R1060A

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX165-HypaCas9

Plasmid

#140567

Purpose

Expresses HypaCas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

N692A M694A Q695A H698A

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX165-evoCas9

Plasmid

#140569

Purpose

Expresses evoCas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

M495V Y515N K526E R661Q

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHHYTK101

Plasmid

#200131

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Su9-MTS (2-96)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK125

Plasmid

#200137

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

OM-Tom70 (2-98)

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK126

Plasmid

#200138

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Pot1-PTS2 (2-50)

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-nLuc

Plasmid

#212611

Purpose

For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-nLuc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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LsgRNA-MS2

Plasmid

#235597

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-Rab5a

Plasmid

#72901

Purpose

Early endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab5a (RAB5A Human)

Use

Lentiviral

Tags

FKBP(F36V) and mCherry

Expression

Mammalian

Mutation

fragment a.a. 2-215

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Mutation

Deleted the partial 3'ribozyme sequence in T…

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_cpCys203

Plasmid

#247759

Purpose

mammalian expression of the cpCys203 FRET-based cysteine sensor

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

cpCys203 Cysteine Sensor

Tags

ECFP and Venus

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP P495L

Plasmid

#62232

Purpose

Mutant of hamster BiP-mGFP unable to bind clients.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Tags

mGFP

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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HA-GFP_rescue_construct

Plasmid

#190641

Purpose

Puromycin-selectable expression of GFP in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

EGFP

Tags

3xHA

Expression

Insect

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB602-mCherry-sfGFP

Plasmid

#213860

Purpose

For Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

Insert

mCherry-sfGFP

Expression

Bacterial and Plant

Promoter

CaMV 35S

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTCW008

Plasmid

#83526

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha2

Expression

Yeast

Promoter

pFUS1J2

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN3

Plasmid

#79257

Purpose

Expresses EGFP-tagged DmPAN3 in S2 cells

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

PAN3 (CG11486 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2

Plasmid

#79256

Purpose

Expresses EGFP-tagged DmPAN2 in S2 cells

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

DmPAN2 (PAN2 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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hs-Cap2

Plasmid

#41972

Depositor

Norbert Perrimon

Article

Baum et al Nat Cell Biol. 2001 Oct;3(10):883-90.

Insert

Cap2 (capt Fly)

Expression

Insect

Promoter

Hsp70

Available Since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
pET29b_cpCys203

Plasmid

#247771

Purpose

bacterial expression of cpCys203 FRET-based cysteine sensor

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

cpCys203 Cysteine Sensor

Tags

ECFP and Venus and His6-TEV

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-split-nLuc

Plasmid

#212612

Purpose

For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-split-nLuc

Use

Luciferase

Expression

Mammalian

Mutation

C373T mutation in CVB3

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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