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pDONR223_RHOA_p.T19N

Plasmid

#82182

Purpose

Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHOA (RHOA Human)

Use

Gateway Cloning

Mutation

T19N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.G12A

Plasmid

#81277

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway Cloning

Mutation

G12A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_B2M_p.A6V

Plasmid

#81263

Purpose

Gateway Donor vector containing B2M , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

B2M (B2M Human)

Use

Gateway Cloning

Mutation

A6V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0-GL-2

Plasmid

#82440

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

Luciferase

Mutation

The complementary region for GL2 siRNA control wa…

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR 19b-3p

Plasmid

#82442

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-19-3p

Mutation

Complementary region for hsa-miR-19-3p as sensor

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,4A5T6T-GFP

Plasmid

#80913

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P4A5T6T and PM,4A5T6T

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGB1-mCRT(P-domain)

Plasmid

#83519

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

Insert

CALR (Calr Mouse)

Tags

GB1 and His

Expression

Bacterial

Mutation

P domain (contains residues 187-283)

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pNP-P+

Plasmid

#81229

Purpose

the CMV promoter of pCS2 has been replaced by a 931-bp fragment of the mitfa promoter and 5'UTR

Depositor

James Lister, David Raible

Article

Lister et al Dev Biol. 2001 Sep 15;237(2):333-44.

Type

Empty backbone

Use

Xenopus//avian/zebrafish

Expression

Mammalian

Promoter

mitfa

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[closed]

Plasmid

#71806

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[closed]

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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MP-P

Plasmid

#69661

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR mutH(E56A)

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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p-super-retro puro RhoGDI2 shRNA

Plasmid

#58897

Purpose

produces an shRNA against RhoGDI2

Depositor

Channing Der

Article

Stevens et al Small GTPases. 2011 Jul;2(4):202-210. Ep

Insert

RhoGDI2 (ARHGDIB Human)

Use

RNAi

Expression

Mammalian

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
p-miR-148a

Plasmid

#65052

Purpose

Expression of miR148a in mammalian cells

Depositor

Cristina Fillat

Article

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

hsa-miR-148a (pre-miRNA) (MIR148A Human)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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p6652 MSCV-P C-Flag HA 8E7-Kozak

Plasmid

#35145

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV8 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
p6647 MSCV-P C-Flag HA 2aE7

Plasmid

#35137

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV2a E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
p6653 MSCV-P C-Flag HA 92E7-Kozak

Plasmid

#35146

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV92 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6654 MSCV-P C-Flag HA 98E7-Kozak

Plasmid

#35148

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV98 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6651 MSCV-P C-Flag HA 76E7-Kozak

Plasmid

#35144

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV76 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6649 MSCV-P C-FlagHA 38 E7-Kozak

Plasmid

#35143

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV38 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6645 MSCV-P C-Flag HA 57E7-Kozak

Plasmid

#35136

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV57 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6656 MSCV-P C-FlagHA 25E7-Kozak

Plasmid

#35022

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV25 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only