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pAc5.1B-EGFP-Dm4E-T-4xmut_T

Plasmid

#147593

Purpose

Insect Expression of Dm4E-T-4xmut

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-4xmut (4E-T Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-5xmut_T

Plasmid

#147594

Purpose

Insect Expression of Dm4E-T-5xmut

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-5xmut (4E-T Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGe1-R1340E-V5His6_G

Plasmid

#146418

Purpose

Insect Expression of DmGe1-R1340E

Depositor

Elisa Izaurralde

Article

Jinek et al RNA. 2008 Oct . 14(10):1991-8.

Insert

DmGe1-R1340E (Ge-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGe1-S1274RQ1277R-V5His6_G

Plasmid

#146419

Purpose

Insect Expression of DmGe1-S1274RQ1277R

Depositor

Elisa Izaurralde

Article

Jinek et al RNA. 2008 Oct . 14(10):1991-8.

Insert

DmGe1-S1274RQ1277R (Ge-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGe1-R1340E-V5His6_F

Plasmid

#146292

Purpose

Insect Expression of DmGe1-R1340E

Depositor

Elisa Izaurralde

Article

Jinek et al RNA. 2008 Oct . 14(10):1991-8.

Insert

DmGe1-R1340E (Ge-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGe1-S1274RQ1277R-V5His6_F

Plasmid

#146293

Purpose

Insect Expression of DmGe1-S1274RQ1277R

Depositor

Elisa Izaurralde

Article

Jinek et al RNA. 2008 Oct . 14(10):1991-8.

Insert

DmGe1-S1274RQ1277R (Ge-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80l AEC

Plasmid

#196473

Purpose

Protein expression of SpyTag-ELP-CarHC

Depositor

Fei Sun

Article

Yang et al Matter (2020) 2, 233–249

Insert

AEC

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC-N-Venus-V150A-CC-Di

Plasmid

#190707

Purpose

Encodes a modified version (V150A) of N-Venus followed by the parallel coiled-coil CC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

N-Venus-V150A-CC-Di

Mutation

V150A

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC-N-Venus-V150A

Plasmid

#190708

Purpose

Control plasmid encoding a modified version (V150A) of N-Venus, but without a coiled-coil to drive reconstitution.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

N-Venus-V150A

Mutation

V150A

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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GB3270

Plasmid

#193114

Purpose

Random sequence R3 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R3

Use

Synthetic Biology

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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GB3269

Plasmid

#193113

Purpose

Random sequence R2 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R2

Use

Synthetic Biology

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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GB2815

Plasmid

#193112

Purpose

Random sequence R1 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R1

Use

Synthetic Biology

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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AAV2/9-shMBVR-HO1-Fd-Fnr-CW3SL

Plasmid

#184003

Purpose

AAV production

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

shMBVR-HO1-Fd-Fnr-CW3SL

Use

AAV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-mad32TAG35TAG

Plasmid

#188999

Purpose

Express madanin-1 with TAG at 32 and 35 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

madanin-1 with TAG at 32 and 35 position

Expression

Bacterial

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pnEK-NvH-DmeIF4E-I96AI112A_Z

Plasmid

#148196

Purpose

Bacterial Expression of DmeIF4E-I96AI112A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4E-I96AI112A

Expression

Bacterial

Mutation

3 silent mutations compared to the sequence given…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnEK-NvSHN-DmeF4E-I96AI112A_Z

Plasmid

#148197

Purpose

Bacterial Expression of DmeIF4E-I96AI112A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4E-I96AI112A

Expression

Bacterial

Mutation

3 silent mutations compared to the sequence given…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Article

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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hp-66%GC-40bp

Plasmid

#169200

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

66%GC-40bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-75%GC-20bp

Plasmid

#169201

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

75%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-75%GC-40bp

Plasmid

#169202

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

75%GC-40bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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