We narrowed to 8,330 results for: gnal

1. pAAV-EF1α1.1-Chronos-GFP

   Plasmid

   #122098

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α (1.1 kb short version)

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCJ199

   Plasmid

   #162672

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO_S.C.V5_NGFR

   Plasmid

   #158331

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   S.C.V5

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO_HSV.Ollas.FLAG_NGFR

   Plasmid

   #158270

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HSV.Ollas.FLAG

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mApple-proα2(I)-G610C

   Plasmid

   #119841

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mApple between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEAHISPApqsE

   Plasmid

   #97019

   Purpose

   Expressing Pseudomonas aeruginosa pqsE protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Quinolone signal response protein
   

   Use

   Tags

   N-ter TEV protease cleavable 6HIs

   Expression

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO_Ollas.C.VSVg_NGFR

   Plasmid

   #158315

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Ollas.C.VSVg

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO_Tag.C.S.Ollas_NGFR

   Plasmid

   #158303

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Tag.C.S.Ollas

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO_HSV.Ollas.C_NGFR

   Plasmid

   #158268

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HSV.Ollas.C

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO_Ollas.C.V5_NGFR

    Plasmid

    #158310

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.C.V5

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO_Ollas.C.FLAG_NGFR

    Plasmid

    #158311

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.C.FLAG

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO_Ollas.C.NWS_NGFR

    Plasmid

    #158312

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.C.NWS

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO_Ollas.C.HA_NGFR

    Plasmid

    #158313

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.C.HA

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO_HA.VSVg.C_NGFR

    Plasmid

    #158263

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.VSVg.C

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO_FLAG.VSVg.C_NGFR

    Plasmid

    #158260

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG.VSVg.C

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid

    #85821

    Purpose

    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    Zf Ubi::cMyc oDam_f_GFPoNLS
    

    Use

    Tol2 based vector

    Tags

    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

    Expression

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTT5-hSDC1-S15A

    Plasmid

    #52363

    Purpose

    expresses human Syndecan-1 serine 15 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S15A and R95Q (according to numbering in Fig. 2A …

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Stx3 triple mutant (F31A D33A E34A)

    Plasmid

    #86945

    Purpose

    Expresses human Stx3 with triple mutation in the apical targeting signal (F31A D33A E34A)

    Depositor

    Thomas Weimbs

    Article

    Soo Hoo et al PLoS One. 2016 Sep 23;11(9):e0163671. do

    Insert

    STX3 triple mutant (F31A D33A E34A) (STX3 Human)
    

    Use

    Tags

    myc-His6

    Expression

    Mammalian

    Mutation

    triple mutant (F31A D33A E34A)

    Promoter

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L4315 IL-10Ra CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244171

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-VP64-ZF6a

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (IL10RA Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    IL10Ra signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244174

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human VEGFR1 SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (FLT1 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    VEGFR1 signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge