We narrowed to 14,585 results for: pET

1. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

   Plasmid

   #62373

   Purpose

   Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
   

   Use

   Tags

   (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…

   Expression

   Bacterial

   Mutation

   Y67S amino acid substitution in (-30)GFP renders …

   Promoter

   T7

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28 His-MBP-TEV-BAP-Human BCKDK

   Plasmid

   #232116

   Purpose

   For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   BCKDK (BCKDK Human)
   

   Use

   Tags

   6 x His, Biotin acceptor peptide (BAP), Maltose-B…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET His6 ProteinG TEV LIC cloning vector (1P)

   Plasmid

   #29660

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, Protein G, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

   Plasmid

   #166421

   Purpose

   Express pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Naegleria fowleri PRL
   

   Use

   Tags

   N-terminal His-tag

   Expression

   Bacterial

   Mutation

   Deleted nucleotides coding for 4 C-terminal amino…

   Promoter

   AmpR, lac1

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMW41: pET-His-MBP-SUMO-R2Bm-SII

   Plasmid

   #209290

   Purpose

   Expresses the R2Bm ORF in E. coli

   Depositor

   Feng Zhang

   Article

   Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

   Insert

   R2
   

   Use

   Tags

   His, MBP, StrepII, and bdSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag

   Plasmid

   #187356

   Purpose

   Bacterial expression and refolding of gp100 pMHC

   Depositor

   Simon Davis

   Article

   Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

   Insert

   hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

   Plasmid

   #114069

   Purpose

   T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a with C-terminal NLS and His-tag
   

   Use

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b/EYFP-GgVcl 884-1066-ECFP FRET

   Plasmid

   #46179

   Depositor

   Susan Craig

   Insert

   Vcl 884-1066 (VCL Chicken)
   

   Use

   Tags

   ECFP, EYFP, and His

   Expression

   Bacterial

   Mutation

   aa 884-1066 (Vt, tail domain)

   Promoter

   T7

   Available since

   July 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His6 Mocr TEV LIC cloning vector (2O-T)

   Plasmid

   #29710

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-Mocr-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET GFP domain 11 LIC cloning vector (GFP C-11)

    Plasmid

    #30183

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), GFP domain 11, and TEV cl…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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