We narrowed to 11,714 results for: NSI

1. pDEST40-2XFL-Src-K298M-Y419F-Y530F

   Plasmid

   #140321

   Purpose

   Expresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   K298M-Y419F-Y530F

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO-GRAB_VIP1.0

   Plasmid

   #208680

   Purpose

   Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tol2 8xSTAR-mNeonGreen. PGK-puro

   Plasmid

   #136255

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mNeonGreen

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TVBB C-term-mNeongreen-Blast

   Plasmid

   #169229

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen-P2A-Blast
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6p1-Rbck1

   Plasmid

   #63135

   Purpose

   Expression of human RBCK1/HOIL-1L

   Depositor

   Titia Sixma

   Article

   Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

   Insert

   HOIL-1L (RBCK1 Human, Synthetic)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Synthetic based on human protein sequence

   Promoter

   tac

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-Ef1a-Con/Foff 2.0-EYFP

   Plasmid

   #137162

   Purpose

   Intersectional viral expression of EYFP in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-SARS2-S-G614 (C-flag)

   Plasmid

   #156421

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF-Con/Fon/Von eYFP

   Plasmid

   #137164

   Purpose

   Intersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV Retrograde and AAV8

   Insert

   3x-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIP-SFFV-mtagBFP-2A STING 368STOP

   Plasmid

   #102590

   Purpose

   Lentivector to express STING delta 368 and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   delta 368

   Promoter

   SFFV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLD-puro-Cn-VA-NHEJ1-WT

    Plasmid

    #141339

    Purpose

    Lentiviral vector for expression of human NHEJ1-WT in mammalian cells

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    NHEJ1 (NHEJ1 Human)
    

    Use

    Lentiviral

    Tags

    VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-nEF-Con/Fon-iC++-EYFP

    Plasmid

    #137155

    Purpose

    Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-iC++-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-ZNF655

    Plasmid

    #88578

    Purpose

    Donor Vector containing ZNF655 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF655 (ZNF655 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-Con/Foff 2.0-oScarlet

    Plasmid

    #137137

    Purpose

    Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    E95D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTWIN1-His6-Ssp-Httex1-43Q

    Plasmid

    #84352

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 43Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-ZNF736

    Plasmid

    #101602

    Purpose

    Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF736 (ZNF736 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-NMHC-IIA-S1916A

    Plasmid

    #101040

    Purpose

    Expresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.

    Depositor

    Tom Egelhoff

    Article

    Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311

    Insert

    Non-muscle myosin IIA heavy chain (MYH9 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S1916A, S1917A

    Promoter

    CMV

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-Con/Fon-sRGECO

    Plasmid

    #137126

    Purpose

    Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-sRGECO
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    E217D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRIP-SFFV-mtagBFP-2A STINGHAQ

    Plasmid

    #102587

    Purpose

    Lentivector to express STING HAQ and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    R71H-G230A-R293Q (HAQ)

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1 KSR2

    Plasmid

    #25968

    Depositor

    Rob Lewis

    Article

    Costanzo-Garvey et al Cell Metab. 2009 Nov . 10(5):366-78.

    Insert

    Kinase suppressor of Ras 2 (Ksr2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pTRIP-SFFV-mtagBFP-2A STING-354STOP

    Plasmid

    #102589

    Purpose

    Lentivector to express STING delta 354 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    delta 354

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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