We narrowed to 11,714 results for: NSI

1. pcDNA3.1-BKa

   Plasmid

   #73213

   Purpose

   Expresses BKa (KCNMA1, mSlo alpha subunit) in mammalian cells. (tagless, leaderless)

   Depositor

   Marcel Bruchez

   Article

   Pratt et al Bioconjug Chem. 2015 Sep 16;26(9):1963-7

   Insert

   BKalpha (Kcnma1 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-Con/Foff 2.0-sRGECO

   Plasmid

   #137127

   Purpose

   Intersectional viral expression of sRGECO in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-sRGECO
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   E217D

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-Coff/Fon-GCaMP6M

   Plasmid

   #137121

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE2-p53DD

   Plasmid

   #196582

   Purpose

   Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   PE2-P2A-mp53DD
   

   Expression

   Mammalian

   Mutation

   Δ40-903

   Promoter

   CMV

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

   Plasmid

   #134653

   Purpose

   lysosome-targeted Sac1 catalytic domain C389S

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
   

   Use

   Lentiviral

   Tags

   FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

   Expression

   Mammalian

   Mutation

   C389S and contains only amino acids 77-520 (Pleas…

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-Coff/Fon-BFP

   Plasmid

   #137131

   Purpose

   Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-Con/Fon-BFP

   Plasmid

   #137129

   Purpose

   Intersectional viral expression of BFP in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223 SARS-CoV-2 S_nostop

   Plasmid

   #152988

   Purpose

   Gateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. MSCV-BRD9-PGK-Puro-IRES-GFP

   Plasmid

   #75114

   Purpose

   Retroviral expression plasmid encoding wild type human BRD9

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Available Since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pRC/CMV-TYK2V678F-VSV

    Plasmid

    #139349

    Purpose

    Expression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)

    Depositor

    Sandra Pellegrini

    Article

    Gakovic et al J Biol Chem. 2008 Jul 4;283(27):18522-9.

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-V678F

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-N2-6MYC-hMID2

    Plasmid

    #51035

    Purpose

    mammalian expression of myc-tagged human MID2

    Depositor

    Timothy Cox

    Article

    Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4

    Insert

    MID2 (MID2 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIP-SFFV-mtagBFP-2A STING-V155M S366A

    Plasmid

    #102593

    Purpose

    Lentivector to express STING V155M S366A and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M S366A

    Promoter

    SFFV

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-HOXD13(+7A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145261

    Purpose

    Mammalian Expression of Nuclear Opto HOXD13(+7A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+7A)-IDR (HOXD13 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVC-DNAJB6b

    Plasmid

    #175065

    Purpose

    expresses mVenus and mCherry tagged DNAJB6b in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    DNAJB6b (DNAJB6 Human)
    

    Tags

    V5 and mVenus + mCherry

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-ZNF574

    Plasmid

    #88644

    Purpose

    Donor Vector containing ZNF574 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF574 (ZNF574 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #135012

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Tags

    3x Flag, 3xFLAG (N terminal on insert), NLS, and …

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTWIN1-His6-Ssp-Httex1-23Q

    Plasmid

    #84349

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. TVBB C-term-mTurquoise2-Hygro

    Plasmid

    #169225

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2-P2A-Hygro
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. R979-X58-008: Hs.KRAS4b(1-188)

    Plasmid

    #159691

    Purpose

    Baculovirus protein expression of KRAS4b (1-188)

    Depositor

    Dominic Esposito

    Article

    Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

    Insert

    Hs.KRAS4b(1-188) (KRAS Human)
    

    Expression

    Insect

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-SARS2-S-D614 (NC-flag)

    Plasmid

    #156418

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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