We narrowed to 16,131 results for: form

1. pcsII-pb2-flag

   Plasmid

   #86766

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   proteasome beta subunit 2 (PSMB2 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-MCS-eDHFR(69K6)-EGFP

   Plasmid

   #172101

   Purpose

   Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   22aa-eDHFR(69K6)-EGFP
   

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SNAP-CLC-EGFP

   Plasmid

   #193579

   Purpose

   Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   Clathrin light chain B (CLTB Human)
   

   Tags

   SNAP-tag and mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTH732-2µ-RLuc/staCFLuc

   Plasmid

   #40607

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The last three codons of the full-length Firefly …

   Promoter

   ADH1 and TDH3

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-human CSF-1R-Y969F,S301-IRES—GFP

   Plasmid

   #115524

   Purpose

   Mammalian expression construct

   Depositor

   Martine Roussel

   Article

   Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

   Insert

   human CSF-1 receptor constitutively activated mutant (CSF1R Human)
   

   Use

   Retroviral

   Mutation

   Y969F,S301

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

   Plasmid

   #170175

   Purpose

   Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2f-ST-P2A-NLS-mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-CMV 3xFLAG-LC3C-GFP

   Plasmid

   #123110

   Purpose

   Expresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3C

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
   

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPGS dnTcf-4(deltaN41)

   Plasmid

   #19284

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   Tcf-4 (TCF4 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Mutation

   Dominant negative, lacks N-terminal 41 amino acids

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pαH-S-RRAR

   Plasmid

   #164569

   Purpose

   SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR variant)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-PRAS40 WT

    Plasmid

    #86758

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    human PRAS40 (AKT1S1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUPER-retro-puro-shOrai1

    Plasmid

    #89933

    Purpose

    Knockdown human Orai1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 shRNA (ORAI1 Human)
    

    Use

    Retroviral

    Available Since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-ms-L-Myc-L351P

    Plasmid

    #50777

    Purpose

    retroviral expression of mouse L-Myc L351P mutant

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    L-Myc-L351P (Mycl Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    L351P (does not bind to Max)

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

    Plasmid

    #104097

    Purpose

    Expresses TREAT-PK co-localization control in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

    Expression

    Mammalian

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLNCX2-STIM1

    Plasmid

    #89817

    Purpose

    Express HUMAN STIM1

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    NO TAG

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-Duet1_6xHis-TEV-GABARAP-Gly

    Plasmid

    #190930

    Purpose

    Plasmid for the expression and purification of GABARAP. Internal Reference: SMC894

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    GABARAP (GABARAP Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Ends with Glycine 116

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBMN Parkin

    Plasmid

    #89299

    Purpose

    For expression of untagged Parkin

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    Parkin (PRKN Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA_B2AR-i-pep

    Plasmid

    #47436

    Purpose

    FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Tags

    10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pYPQ265E6

    Plasmid

    #213469

    Purpose

    Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

    Depositor

    Yiping Qi

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Insert

    TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
    

    Use

    CRISPR; Gateway compatible tada-cda-32aa linker-z…

    Tags

    No

    Expression

    Plant

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

    Plasmid

    #163979

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSB116 - pL2_pSB90_fLUC-I_P19

    Plasmid

    #123191

    Purpose

    binary plant vector for transient TBSV P19 and fLUC (with intron) expression

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::P19::tNOS 2x35S::fLUC-I::tNOS
    

    Use

    Luciferase

    Expression

    Plant

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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