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pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

Plasmid

#175417

Purpose

Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 R124A/K125A nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

R124A and K125A

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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R619-M67-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6

Plasmid

#166018

Purpose

mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD)

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S )

Tags

His6

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKTop::tse5-CT (1169-1281)

Plasmid

#192955

Purpose

For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT (1169-1281)

Expression

Bacterial

Mutation

encodes for tse5 (1169-1281)

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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Nsp13 deltaN1-EGFP

Plasmid

#165127

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN1 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp13 deltaN2-EGFP

Plasmid

#165128

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp14D-EGFP

Plasmid

#165125

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp14D (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

Plasmid

#184406

Purpose

yeast surface display of the SARS-CoV-2 Beta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

K417N+E484K+N501Y

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167848

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Tags

6xHis and AviTag

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET-30a-NSP14-SARS-CoV

Plasmid

#236424

Purpose

bacterial protein expression of NSP14

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP14 (ORF1ab SARS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP14-MERS-CoV

Plasmid

#236425

Purpose

bacterial protein expression of NSP14

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP14 (orf1ab MERS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP10-SARS-CoV-2

Plasmid

#236426

Purpose

bacterial protein expression of NSP10

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP10 (ORF1ab SARS-CoV-2)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP10-SARS-CoV

Plasmid

#236427

Purpose

bacterial protein expression of NSP10

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP10 (ORF1ab SARS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP10-MERS-CoV

Plasmid

#236428

Purpose

bacterial protein expression of NSP10

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP10 (orf1ab MERS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S-ectodomain

Plasmid

#235984

Purpose

Internally tagged SARS-CoV-2 spike ecto-domain

Depositor

Syed Saif Hasan

Article

Singh et al J Struct Biol X. 2025 Feb 11;11:100123.

Insert

Spike protein (S SARS-CoV-2)

Tags

8xHis and Twin-Strep tag and internal Twin-Strep …

Expression

Mammalian

Mutation

aa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pINQ-ON10

Plasmid

#198689

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, HA tag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pINQ-NbH4

Plasmid

#198690

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody H4

Tags

6xHis tag, Avitag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-crCoV-Mutiplex_EF1a-BFP

Plasmid

#224788

Purpose

SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crCoV20, crCoV21, crCoV24

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6 and hU6-2xTetO

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)

Plasmid

#194233

Purpose

Mammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domain

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

CD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258 (S Synthetic)

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-852

Plasmid

#218010

Purpose

Receptor binding domain of SARS-CoV-2

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

RBD (S SARS-CoV-2)

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-850

Plasmid

#218005

Purpose

Receptor binding domain of SARS-CoV-2

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

RBD (S SARS-CoV-2)

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge