We narrowed to 12,356 results for: nsf

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pAAV-CaMKII-GCaMP6f-4x128-mAGNET

Plasmid

#166023

Purpose

Expresses GCaMP6f in CaMKII+ cells with low miRNA-128 expression

Depositor

Xue Han

Article

Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

Insert

GCaMP6f with 4 miR-128 binding sites

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTOPO-HqERV(del5'-LTR)

Plasmid

#164089

Purpose

transfection of Hq-ERV without 5'-LTR

Depositor

Maik Hintze

Article

Hintze et al Mamm Genome. 2020 Dec 26. pii: 10.1007/s

Insert

Hq-ERV

Use

Eukaryotic expression vector

Mutation

deletion of 5'-LTR

Promoter

none (5'-LTR deleted)

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTOPO-HqERV(C1-20)

Plasmid

#164090

Purpose

transfection of Hq-ERV with light-inducible promoter

Depositor

Maik Hintze

Article

Hintze et al Mamm Genome. 2020 Dec 26. pii: 10.1007/s

Insert

Hq-ERV

Use

Eukaryotic expression vector

Mutation

5'-LTR replaced with light-regulatable C1-20…

Promoter

light-activatable C1-20

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE669

Plasmid

#153235

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-LAP1-mCherry

Plasmid

#159525

Purpose

Expresses mCherry from LAP1 promoter

Depositor

Esteban Engel

Article

Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

LAP1

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE672

Plasmid

#153238

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mIsl2-EGPF

Plasmid

#153191

Purpose

Mouse Isl2 (mIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-CBD-hGLY

Plasmid

#134596

Purpose

Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

human glycine acyltransferase 2 (GLYATL2 )

Tags

chitin binding domain

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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D70_Ld_210610

Plasmid

#135883

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

dihydrolipoamide acetyltransferase precursorlike protein

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-GalT-mKate2

Plasmid

#109506

Purpose

Muscle specific zebrafish expression of mKate2 fused to the N-terminal sequence from zebrafish 1,4 galactosyltransferase 1 (GalT). Marks the Golgi complex.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-GalT-mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Thy1.1-muSOX D219A

Plasmid

#131704

Purpose

encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

Depositor

Britt Glaunsinger

Article

Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

Insert

Thy1.1-muSOX

Expression

Mammalian

Mutation

Aspartic acid 219 mutated to an alanine

Promoter

CMV

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-mCherry-FHA1-subT391A-mCherry

Plasmid

#104462

Purpose

Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

Depositor

Bernardo Sabatini

Article

Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

Insert

mCherry-subT391A-FHA1-mCherry

Use

AAV

Tags

mCherry

Promoter

CAG

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMflT-o1

Plasmid

#101309

Purpose

Contains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Insert

oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-Zeo-NKX2-8

Plasmid

#75090

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

NKX2-8 (NKX2-8 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_Golgi

Plasmid

#65694

Purpose

Expresses Golgi-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

N-terminal 81 amino acids of beta 1,4-galactosyltransferase

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_Golgi

Plasmid

#65693

Purpose

Expresses Golgi-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

N-terminal 81 amino acids of beta 1,4-galactosyltransferase

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2 YFP-Smo-Weyer'sAla5

Plasmid

#41016

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

smoothened homolog (Smo Mouse)

Tags

EVC2 ala5 (aa 1177-1220 with amino acids 1185-118…

Expression

Mammalian

Mutation

YFP inserted after Smo signal sequence. Transfer…

Available Since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pAAVf-EnhCB-lacZnls mir-122 1xBS

Plasmid

#35643

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

1 miR-122 target site

Use

AAV

Promoter

CB

Available Since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-NE2m

Plasmid

#208686

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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RDX-mCherry

Plasmid

#214476

Purpose

Plasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.

Depositor

Natalie Ahn

Article

Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

Insert

RDX (RDX Human)

Tags

mCherry

Expression

Mammalian

Mutation

Sequence matches cDNA clone MGC:48283 IMAGE:52844…

Promoter

CMV

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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