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pMIH-mRuby2-BAK

Plasmid

#111627

Purpose

Fluorescent fusion protein used to visualise mouse BAK, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

BAK (Bak1 Mouse)

Use

Retroviral

Tags

mRuby2

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIP5K1A

Plasmid

#23755

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5K1A (PIP5K1A Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKAA1

Plasmid

#23871

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAA1 (PRKAA1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKCG

Plasmid

#23640

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCG (PRKCG Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-EGR3-P2A-mCherry

Plasmid

#203861

Purpose

Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

EGR3 (EGR3 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-PLXNA3

Plasmid

#23518

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PLXNA3 (PLXNA3 Human)

Use

Gateway donor vector

Mutation

L1655M

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SRPK2

Plasmid

#23766

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SRPK2 (SRPK2 Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pYX-C-mScarlet-Zeo-TSC22D2

Plasmid

#222670

Purpose

endogenously tag TSC22D2 with C-terminal mScarlet

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Tags

mScarlet

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PRKCD

Plasmid

#20603

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKCD (PRKCD Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-TWF2

Plasmid

#23700

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TWF2 (TWF2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKD2

Plasmid

#23490

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKD2 (PRKD2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRPS2

Plasmid

#23622

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRPS2 (PRPS2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pYO1025

Plasmid

#235705

Purpose

Expression of FL human VPS34 and human VPS15-ZZ in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Inserts

VPS34 (PIK3C3 Human)
VPS15 (PIK3R4 Human)

Tags

3xTEV-ZZ

Expression

Mammalian

Promoter

CAG and CMV

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pIG-874_HA-GD2-28z_CAR_IL2RA

Plasmid

#207504

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

IL2RA, HA-GD2-28z_CAR (IL2RA Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PB-PE SpRY

Plasmid

#179082

Purpose

NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…

Promoter

CAG

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-671_HA-GD2-28z_CAR_tNGFR

Plasmid

#207484

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_AMBRA1#1

Plasmid

#174152

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-STK32C

Plasmid

#23720

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK32C (STK32C Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKG2

Plasmid

#23435

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKG2 (PRKG2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pBabe-Neo-Myr-Flag-AKT1

Plasmid

#15266

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Tags

Myr-Flag

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only