We narrowed to 15,733 results for: LEA

1. pCMV-T7-ABE8e-SpCas9(KWRQLC)-P2A-EGFP (RAS3467)

   Plasmid

   #223112

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(KWRQLC)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/KWRQLC)=D1135K, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-ABE8e-SpCas9(MRKCRS)-P2A-EGFP (BKS1034)

   Plasmid

   #223117

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MRKCRS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MRKCRS)=D1135M, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR8/GW_TOPO_Cdk13_Nterm_FlagHa

   Plasmid

   #127343

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Cyclin Dependent Kinase 13 (Cdk13 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   Flag- HA- Tandem Epitopes

   Mutation

   Base pairs 1-1554 of transgene are codon optimize…

   Promoter

   None

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-Neo-TetO-Cdk13_Untagged

   Plasmid

   #127344

   Purpose

   Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Cyclin Dependent Kinase 13 (Cdk13 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   None

   Expression

   Mammalian

   Mutation

   Base pairs 1-1554 of transgene are codon optimize…

   Promoter

   TetO Promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-Neo-TetO-Cdk13_Nterm_FlagHa

   Plasmid

   #127345

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Cyclin Dependent Kinase 13 (Cdk13 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   Flag- HA- Tandem Epitopes

   Expression

   Mammalian

   Mutation

   Base pairs 1-1554 of transgene are codon optimize…

   Promoter

   TetO Promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]

   Plasmid

   #196887

   Purpose

   Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16

   Plasmid

   #210506

   Purpose

   expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16 component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn-PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP

   Plasmid

   #210509

   Purpose

   expresses PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP component in rat hippocampal neuron cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Inserts

   hSyn promoter (RIC8B )
   PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pαH-S-GSAS-2P-N317P

   Plasmid

   #196378

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

   Depositor

   Priyamvada Acharya

   Article

   Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:

   Insert

   S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   HRV 3C cleavage site (before tags) C terminal on …

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378)

    Plasmid

    #185484

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(SCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-EGFP (LTH1441)

    Plasmid

    #185486

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(PCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-NLS(SV40)-anCas(FCA)-BPNLS(SV40)-P2A-EGFP (LTH1380)

    Plasmid

    #185487

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(FCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

    Plasmid

    #185489

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid

    #185492

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. t1-405(del30)

    Plasmid

    #166128

    Purpose

    Expression of human talin-1 head (residues 1-405) in E. coli. Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    T1head1-405(del30) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 1-405, with 30 amino acid deletion in th…

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. t1-405(T144E,T150E)

    Plasmid

    #166130

    Purpose

    For expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    HuT1head1-405(T144E,T150E) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 1-405, mutations T144E and T150E

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. px330 Gatad2a sgRNA (for deletion)

    Plasmid

    #110814

    Purpose

    Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

    Depositor

    Jacob Hanna

    Article

    Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

    Insert

    mGataD2a (Gatad2a Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4.23-CD69 CaRE1 (minus strand)

    Plasmid

    #91842

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0619)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE1 (minus strand) (CD69 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4.23-IL2RA CaRE6 (minus strand)

    Plasmid

    #91840

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0617)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE6 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    rs7893467 C>A

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4.23-IL2RA CaRE6 (plus strand)

    Plasmid

    #91839

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0616)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE6 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    rs7893467 G>T, rs11256448 A>G

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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