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pEM-Cas9noSsrA

Plasmid

#102285

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Mutation

removed ssrA tag

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-CenpB 1-166-Sgo1 N61I

Plasmid

#108498

Purpose

expression of vsv-CenpB 1-166-Sgo1 N61I (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Tags

VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1, no PP2A binding N…

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pScalps_puro_mklf1

Plasmid

#59918

Purpose

Expresses mouse Kruppel-like factor1

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

Kruppel-like factor 1 (Klf1 Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
p5M-PR-B-1-688

Plasmid

#89115

Purpose

mammalian expression of human progesterone receptor amino acid residues 1-688 in pCMV5 with 5 restriction sites deleted in polylinker

Depositor

Elizabeth Wilson

Article

Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

Insert

human progesterone receptor-B N-term+DNA binding domain (PGR Human)

Expression

Mammalian

Mutation

5 restriction sites deleted in polylinker, contai…

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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mCardinal-Cx43-7

Plasmid

#56157

Purpose

Localization: Gap Junctions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Cx43

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pHL417

Plasmid

#62822

Purpose

Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::micC::Asp terminator + pLtetO-1::RBS (st7) hfq::T1 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

gfp
micC leader
hfq leader

Use

Synthetic Biology

Tags

ompC leader and st7 RBS

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-139-5p-Decoy.hPGK.GFP.WPRE

Plasmid

#46588

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-139-5p Decoy (MIR139, Mir139 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-127-Decoy.hPGK.GFP.WPRE

Plasmid

#46583

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-127 Decoy (MIR127, Mir127 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pPK1E-2+t-gfp

Plasmid

#48132

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-130b-Decoy.hPGK.GFP.WPRE

Plasmid

#46586

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-130b Decoy (MIR130B, Mir130b Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-100-Decoy.hPGK.GFP.WPRE

Plasmid

#46580

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-100 Decoy (MIR100, Mir100 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
Tip alpha K102A/K104A (21-192)

Plasmid

#23159

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Tags

His

Expression

Bacterial

Mutation

changed lysine 102 and lysine 104 to alanine, and…

Available Since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-431-Decoy.hPGK.GFP.WPRE

Plasmid

#46620

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-431 Decoy (MIR431, Mir431 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pSTTa-GNAI1

Plasmid

#248651

Purpose

Vector for protein expression of 2x StrepII-tagged human heterotrimeric G protein GNAI1 in E. coli.

Depositor

Sarah Assmann

Article

Gookin et al Front Mol Biosci. 2025 Apr 7;12:1513660.

Insert

GNAI1 (GNAI1 Human)

Tags

2x StrepII, Thrombin site, TEV site

Expression

Bacterial

Promoter

tac

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pIVT_goldDn29-P2A-GFP

Plasmid

#247163

Purpose

In vitro transcription of human codon optimized engineered goldDn29-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-P2A-GFP

Use

In vitro transcription

Tags

6xHis

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pWidom601.CDKN2Bp.601

Plasmid

#247019

Purpose

Two Widom601 sequences, with a short linker with sequence from the CDKN2B locus between them. For large scale PCR amplification of DNA for nucleosome refolding.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Widom601 sequences and CDKN2B locus

Use

Synthetic Biology

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-pYtag(Scarlet)-ScarlessDsRed

Plasmid

#244938

Purpose

Vector containing a Scarlet-based pYtag biosensor for CRISPR insertion into Drosophila RTKs, with a DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

mScarlet-tagged pYtag system (3xITAM P2A mScarlet-ZtSH2)

Use

CRISPR

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2CR-QUAS:NTR-mKate2

Plasmid

#241914

Purpose

Zebrafish transgenesis plasmid encoding nitroreductase-mKate2, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker

Depositor

Balázs Enyedi

Article

Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

Insert

QUAS enhancer driving nitroreductase-mKate2

Use

Zebrafish tol2 transgenesis

Tags

nitroreductase fused with mKate2

Promoter

QUAS

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-LSL-Cyp26b1-PGK-H2BRFP

Plasmid

#241862

Purpose

Cre-mediated Cyp26b1 overexpression

Depositor

Elaine Fuchs

Article

Tierney et al Science. 2024 Mar 8;383(6687):eadi7342.

Insert

Cyp26b1 (Cyp26b1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-2

Plasmid

#237891

Purpose

Cre-KO AAV vector#2 containing SaCas9 and its sgRNA targeting Cre

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

sgRNA-Cre

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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