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pEGFP-C3-PLK4-S305E-3xFLAG

Plasmid

#69840

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305E

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pHis-zfCSF3b

Plasmid

#65613

Purpose

express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

Insert

Csf3b (csf3b Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only
Tmem214 pEGFP-N2 (1072)

Plasmid

#62026

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
Tmem38A pEGFP-N2 (1401)

Plasmid

#62050

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem38A (TMEM38A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pMG

Plasmid

#58991

Purpose

MSCV based expression vector - expresses GFP

Depositor

David Baltimore

Article

So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

Type

Empty backbone

Use

Retroviral; Mscv-based retroviral system that exp…

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Xpress beta4 1355T

Plasmid

#16040

Depositor

Filippo Giancotti

Article

Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

Insert

beta4 (ITGB4 Human)

Tags

6xHis and Xpress

Expression

Mammalian

Mutation

1355T: amino acids 1-1355

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
Tapasin-TM

Plasmid

#254045

Purpose

psffv-tapasin-TM-FLAG

Depositor

Nikolaos Sgourakis

Article

Hwang et al Sci Adv. 2026 Jan 2;12(1):eaeb0821. doi:

Insert

Tapasin-TM (TAPBP Human)

Use

Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Mutation

removed C terminal domain, replaced with that of …

Promoter

sffv

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pYTRW22K_7Ti1

Plasmid

#177293

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZ

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

lacZ

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW09K_0T5

Plasmid

#177281

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

Ptet-tetA(C)

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTarget_AT1R-tTA

Plasmid

#222851

Purpose

Mammalian expression plasmid encoding the human Angiotensin II type I receptor fused to the tTA transcriptional activator

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT1R (AGTR1 Human)

Tags

FLAG and tTA

Expression

Mammalian

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBC155

Plasmid

#202274

Purpose

Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc182

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-F170C

Plasmid

#125848

Purpose

express mutant BAP1-F170C

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

F170C

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-G109V

Plasmid

#125846

Purpose

express mutant BAP1-G109V

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

G109V

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pUDR211

Plasmid

#113870

Purpose

expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA3-HXT8 / sgRNA4-HXT14

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUDR418

Plasmid

#113875

Purpose

expression of double Cas9 programming sgRNA11 targetting STL1

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

doble sgRNA11-STL1

Use

CRISPR

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMTgR2

Plasmid

#107286

Purpose

Plasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.

Depositor

Bohdan Schneider

Article

Mikulecky et al Acta Crystallogr D Struct Biol. 2016 Sep

Insert

Interferon gamma receptor 2 (IFNGR2 Human)

Tags

CATCATCACCATCACCATGA

Expression

Insect

Promoter

Metallothionein promoter

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pKDEL-mScarlet3-H (aka mYongHong)

Plasmid

#239411

Purpose

Expresses mScarlet3-H (aka mYongHong) localized to ER in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

mScarlet3-H-KDEL

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-OMICRON

Plasmid

#180423

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS

Depositor

Priyamvada Acharya

Article

Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

Insert

Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_RB1#2

Plasmid

#174151

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

RB1 (RB1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His10FLAG

Plasmid

#157736

Purpose

empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Article

Olson et al Protein Expr Purif. 2021 May 7:105890. d

Type

Empty backbone

Tags

His10 + 3xFLAG, enterokinase-cleavable

Expression

Insect

Promoter

polyhedrin

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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