173,763 results
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Plasmid#183193PurposePlasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6, mU6Available SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pHS_Nova
Plasmid#239831PurposeNovaIscB mammalian expressionDepositorInsertNovaIscB
TagsNucleoplasmin NLS-3xHA and SV40 NLSExpressionMammalianMutationInsertion of REC domain from NbaCas9-1 and swap o…PromoterCMVAvailable SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 S727A
Plasmid#12304DepositorAvailable SinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHR-iRFP670-CaaX
Plasmid#170464PurposeExpresses iRFP670 fluorophore targeted to the plasma membrane using a prenylation motifDepositorInsertiRFP670-CaaX
UseLentiviralTagsCaaXExpressionMammalianPromoterSFFVAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-S33Y Beta-catenin
Plasmid#19286PurposeMammalian expression of human beta-catenin with Flag tag and S33Y mutationDepositorInsertbeta-catenin (CTNNB1 Human)
TagsFlagExpressionMammalianMutationS33Y (presumptive GSK3β phosphorylation site)Available SinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-hCD8
Plasmid#153417PurposeNFAT-driven ZsGreen-1 reporter gene with human CD8ab geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
UseRetroviralExpressionMammalianAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
scAAV-Cre
Plasmid#177933PurposeExpresses Cre-recombinase and can be packaged into AAV particles.DepositorInsertCAG-Cre
UseAAVExpressionMammalianPromoterU6Available SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP
Plasmid#175521PurposeNuclear expression of HaloTag10 in mammalian cellsDepositorInsertH2B-HaloTag10
ExpressionMammalianMutationHaloTag10 = HaloTag7-Q165WAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (LaG17)
Plasmid#188380PurposeLentiviral vector - constitutive expression of an anti-GFP SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiGFP_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only