We narrowed to 19,334 results for: Kin

1. pCDF-NtXNtS1R1R2B2

   Plasmid

   #160882

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcS-S1, rbcX, raf1, raf2, bsd2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rbcS-S1: T7, chaperones: T7

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRXG

   Plasmid

   #113643

   Purpose

   This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   mRFP1-linker-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fast-GCaMP-EF19

   Plasmid

   #52644

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-EF19
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T328D/T330D mutated from GCaMP3

   Promoter

   T7

   Available since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pEN_TT 3xFLAG Luciferase

   Plasmid

   #98391

   Purpose

   Gateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   Luciferase
   

   Use

   Entry vector for gateway cloning

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   TRE tight and TRE tight promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AY42_pS.Donor.R5

   Plasmid

   #100291

   Purpose

   CCR5-targeting plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   18 bp RFLP (MluI+KspAI)
   

   Use

   CRISPR and Synthetic Biology; Human targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS NL4-3 envFS eGFP RT-D185K/D186L

   Plasmid

   #101332

   Purpose

   pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 envFS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   reverse transcriptase D185K/D186L

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN-PITCh-FAID

   Plasmid

   #127885

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-H3F-AID (PPP2CA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ki67∆repeats-mNeonGreen-IRESpuro2

   Plasmid

   #183742

   Purpose

   Expresses Ki67∆repeats-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67(lacking repeats) (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67(lacking repeats)

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE- TRE-DEST-NBioTAP

   Plasmid

   #53568

   Purpose

   Lentiviral destination vector for N -Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

   Depositor

   Mitzi Kuroda

   Article

   Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-TRX

    Plasmid

    #21615

    Depositor

    John Kyriakis

    Article

    Liu et al Mol Cell Biol. 2000 Mar . 20(6):2198-208

    Insert

    thioredoxin (TXN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

11. HS-PCNA-GFP

    Plasmid

    #105942

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. JH03

    Plasmid

    #73512

    Purpose

    mKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mKate2::PH::tbb-2
    

    Use

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    mex-5

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. YSEHPTFTSQY_epitope

    Plasmid

    #165016

    Purpose

    Expression of CMV UL83 (363-373) fused to IL-2 signal sequence

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    CMV UL83 (363-373)
    

    Use

    Lentiviral

    Tags

    human IL2 signal sequence at N-term

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD/HisB-Medium-FT

    Plasmid

    #31917

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    araB

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. 817 pSG5L HA PTEN G129R

    Plasmid

    #10748

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G129R

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pJFRC-20X-UAS-ADARcd

    Plasmid

    #81173

    Purpose

    identification of cell-specific targets of RNA binding proteins

    Depositor

    Michael Rosbash

    Article

    McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

    Insert

    UAS-ADARcd
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBSFI-p110δ

    Plasmid

    #34893

    Depositor

    Peter Vogt

    Article

    Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

    Insert

    p110δ (PIK3CD Human)
    

    Use

    Adaptor plasmid

    Tags

    Expression

    Mutation

    See depositor comments below.

    Promoter

    Available since

    Feb. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX4T1-TRAF6-C70A

    Plasmid

    #43930

    Depositor

    Bryant Darnay

    Article

    Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

    Insert

    TNF Receptor-Associated Factor 6 (Traf6 )
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C70A

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS NL4-3 envFS eGFP Δnef/U3

    Plasmid

    #101346

    Purpose

    Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta nef/LTR

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLIK_hygro_BirA

    Plasmid

    #136537

    Purpose

    Lentiviral expression vector for an inducible BirA

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    BirA
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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