We narrowed to 12,778 results for: NUC

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pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P

Plasmid

#147209

Purpose

Insect Expression of DmXRN1-P1339AP1342A-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1-P1339AP1342A-dsRNAres (pcm Fly)

Expression

Insect

Mutation

one silent mtuation compared to the sequence give…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-S12AQ18AH19A_V

Plasmid

#147805

Purpose

Mammalian Expression of HsDCP1a-S12AQ18AH19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-S12AQ18AH19A (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmx4-V5 C209A

Plasmid

#175140

Purpose

Lentiviral expression of V5-tagged mouse Tmx4

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

C209A

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmx4-V5 C211A

Plasmid

#175139

Purpose

Lentiviral expression of V5-tagged mouse Tmx4

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

C211A

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Hsd11b1-V5

Plasmid

#175147

Purpose

Lentiviral expression of mouse Hsd11b1-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Hsd11b1 (Hsd11b1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmx4-V5 C209A, C211A

Plasmid

#175141

Purpose

Lentiviral expression of V5-tagged mouse Tmx4

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

C209A, C211A

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(K206A/K244A)

Plasmid

#177135

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_2_dCas9_VP64_sgRNA

Plasmid

#163707

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VP64+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET41sIII_GST-ALC1-K77R

Plasmid

#174813

Purpose

Bacterial expression of inactive ALC1 (K77R disrupts ATP binding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1-K77R (CHD1L Human)

Tags

GST

Expression

Bacterial

Mutation

K77R

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET41sIII_GST-ALC1-E175Q

Plasmid

#174814

Purpose

Bacterial expression of inactive ALC1 (E175Q disrupts ATP binding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1-E175Q (CHD1L Human)

Tags

GST

Expression

Bacterial

Mutation

E175Q

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC-N-Myc-Flag-BirA-hPOT1-DeltaOB

Plasmid

#166410

Purpose

express BirA-hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

POT1 ΔOB (POT1 Human)

Tags

MYC-flag-BirA

Expression

Mammalian

Mutation

ΔOB

Promoter

cmv

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGA_pET28

Plasmid

#167326

Purpose

Expresses TFAM HMGA mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA domain (43-122)

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGA_linker_pET28

Plasmid

#167327

Purpose

Expresses TFAM HMGA+linker mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA and linker doma…

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGB_ctail_pET28

Plasmid

#167330

Purpose

Expresses TFAM HMGB+C-tatil mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGB and C-tail doma…

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_dC_pET28

Plasmid

#167328

Purpose

Expresses TFAM dC-tail mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA, linker and HMG…

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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LR (hsp70I: cPLA2-mK2)

Plasmid

#162564

Purpose

Measuring cPLA2 activation in live cells after heat shock.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Inserts

Heat shock protein 70 (HSPA1A Human)
cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Gateway donor vector

Tags

mK2

Promoter

cmlc2

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-dt/CBA-EGFP-NLS

Plasmid

#163918

Purpose

Contains bidirectional reporter genes dtomato and nuclear-targeting EGFP.

Depositor

John Engelhardt

Article

Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

Insert

dtomato and EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

Added nuclear localization signal to EGFP

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 TNos:Luc:5'UTR (GB1499)

Plasmid

#160580

Purpose

Reversed sequences of the NOS terminator, Firefly Luciferase CDS and 5' UTR of 35S promoter

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

Luc

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 PhiC31 integrase (GB1561)

Plasmid

#160586

Purpose

Streptomyces phage PhiC31 integrase, adapted for C-terminal fusions

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 linker-RDF (GB2892)

Plasmid

#160604

Purpose

RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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