We narrowed to 8,596 results for: Set

1. pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct

   Plasmid

   #175554

   Purpose

   Targeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   NANOG
   

   Use

   Mouse Targeting

   Tags

   FKBPV-HA2-P2A-eGFP

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

   Plasmid

   #154196

   Purpose

   Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sgRNA-BC1-A, sgRNA-BC1-C
   

   Use

   Lentiviral; Catch barcode activation

   Expression

   Mammalian

   Promoter

   hU6 and mU6

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcU6_3 MS2 sgRNA

   Plasmid

   #92394

   Purpose

   Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chick U6.3

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-mNeon-Puro-TOMM20

   Plasmid

   #207790

   Purpose

   Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TOMM20 Homology Arms flanking a mNeon-Puro Cassette (TOMM20 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet-1_6xHis-TEV-mCh-NDP52

   Plasmid

   #187829

   Purpose

   Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NDP52 (CALCOCO2 Human)
   

   Tags

   6xHisTag, TEV, and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV.EF1α.mEmerald.CD9.miR9T

   Plasmid

   #170452

   Purpose

   Lentiviral plasmid that encodes mEmerald-CD9 fusion protein with microRNA 9 tandem cassette to restrict expression to microglia with EF1-alpha promoter.

   Depositor

   Tsuneya Ikezu

   Article

   Clayton et al Mol Neurodegener. 2021 Mar 22;16(1):18.

   Insert

   CD9 (CD9 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Promoter

   EF1a

   Available Since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82704

   Purpose

   AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   H1/TO-L Empty gRNA Expression Cassette
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

   Plasmid

   #154197

   Purpose

   Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sgRNA-BC2-A, sgRNA-BC2-C
   

   Use

   Lentiviral; Catch barcode activation

   Expression

   Mammalian

   Promoter

   hU6 and mU6

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Blast-3xFLAG-LMNB1

   Plasmid

   #207777

   Purpose

   Donor template for Blast-2A-3xFLAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-3xFLAG Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. qTAG-C-mStayGold-Puro-CLTC

    Plasmid

    #227314

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82703

    Purpose

    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-Coff/Fon-GCaMP6M

    Plasmid

    #137121

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMDC32B-BS-AtMIR390a-B/c

    Plasmid

    #199560

    Purpose

    Plant expression vector (2x35S) for direct cloning of amiRNAs into Arabidopsis thaliana MIR390a precursor including only the basal stem

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2023 Oct 27;51(19):10

    Insert

    Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
    

    Use

    RNAi

    Promoter

    2x35S

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPC1000

    Plasmid

    #174829

    Purpose

    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Inserts

    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    EF1a and mouse U6

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-N-Blast-3xHA-LMNB1

    Plasmid

    #207778

    Purpose

    Donor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-3xHA Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pRDA_122

    Plasmid

    #208095

    Purpose

    Perturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seq

    Depositor

    John Doench

    Article

    Geiger-Schuller et al bioRxiv. 2023 Jan 24:2023.01.23.525198.

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Promoter

    EF1a

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82702

    Purpose

    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    H1/TO Empty gRNA Expression Cassette
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pROF346

    Plasmid

    #155331

    Purpose

    Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    RB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBUN501

    Plasmid

    #50582

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    zCas9D10A
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Mutation

    Cas9D10A nickase, was derived from the zCas9 and …

    Promoter

    AtU6-26p and Ubi1p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. INS-GFP-CD19-EPT

    Plasmid

    #210469

    Purpose

    Donor plasmid for knock-in GFP-CD19 into the human INS locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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