Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 25,310 results for: expression vector

Showing: 8161 - 8180 of 25310 results

  1. pGEX6P1-N-Myc

    Plasmid
    #119757
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.
    Depositor
    Martin Reijns, Andrew Jackson
    Type
    Empty backbone
    Use
    Tags
    GST and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119757-plasmid-map-sequence-id-233668

    Enlarge

  2. 3xFlag-CEP83

    Plasmid
    #200442
    Purpose
    Expresses 3xFlag-tagged mouse CEP83 in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    CEP83 (CCDC41 Human)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    200442-plasmid-map-sequence-id-408797

    Enlarge

  3. LSL-Cas9-Rosa26TV

    Plasmid
    #61408
    Purpose
    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    Cas9
    EGFP
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    CAG
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    61408-plasmid-map-sequence-id-333613

    Enlarge

  6. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    99154-plasmid-map-sequence-id-352449

    Enlarge

  7. pUBC-SV40NLS-Pfv-Sapphire

    Plasmid
    #203653
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203653-plasmid-map-sequence-id-402484

    Enlarge

  8. pUBC-Pfv-Sapphire_PURO

    Plasmid
    #203651
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203651-plasmid-map-sequence-id-402481

    Enlarge

  9. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid
    #106964
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    106964-plasmid-map-sequence-id-207987

    Enlarge

  10. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid
    #106965
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106965-plasmid-map-sequence-id-207982

    Enlarge

  11. pDEST51-LRRK2-G2019S

    Plasmid
    #29401
    Depositor
    Mark Cookson
    Insert
    LRRK2 (LRRK2 Human)
    Use
    Destination vector
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Gly2019Ser
    Promoter
    Available since
    April 1, 2011
    Availability
    Academic Institutions and Nonprofits only
    29401-plasmid-map-sequence-id-256667

    Enlarge

  12. pDEST51-LRRK2-R1398Q

    Plasmid
    #29398
    Depositor
    Mark Cookson
    Insert
    LRRK2 (LRRK2 Human)
    Use
    Destination vector
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Arg1398Gln
    Promoter
    Available since
    April 1, 2011
    Availability
    Academic Institutions and Nonprofits only

  13. pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo

    Plasmid
    #128453
    Purpose
    lentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128453-plasmid-map-sequence-id-261484

    Enlarge

  14. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid
    #99248
    Purpose
    single-vector system with EGFP reporter for use in CROPseq knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99248-plasmid-map-sequence-id-191832

    Enlarge

  17. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192665
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192665-plasmid-map-sequence-id-379871

    Enlarge

  18. RV-Syn-Dio-yfp_2a_elfn1

    Plasmid
    #98177
    Purpose
    Retroviral vector encoding Cre-dependent expression of EYFP-P2A-elfn1
    Depositor
    Oscar Marin
    Insert
    Elfn1 (Elfn1 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Syn
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    98177-plasmid-map-sequence-id-183380

    Enlarge

  19. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

    Plasmid
    #192666
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192666-plasmid-map-sequence-id-379913

    Enlarge

  20. pSMPUW_6.19_Ex_Ef1a-EGFP-HA-SV40-Zeo

    Plasmid
    #128455
    Purpose
    lentiviral expression vector with Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128455-plasmid-map-sequence-id-256467

    Enlarge

Showing: 8161 - 8180 of 25310 results