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We narrowed to 16,006 results for: grna

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Showing: 8161 - 8180 of 16006 results

  4. pU6T7G

    Plasmid
    #71463
    Purpose
    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)
    Depositor
    Willie Mark
    Publication
    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    human U6/phage T7
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71463-plasmid-map-sequence-id-128761

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  5. PX458_ZNF219_1

    Plasmid
    #72368
    Purpose
    Encodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZNF219 (ZNF219 Human)
    Use
    CRISPR
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72368-plasmid-map-sequence-id-132118

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  10. pX330 EGFR-sgRNA

    Plasmid
    #188633
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR
    Depositor
    Jared Toettcher
    Publication
    Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    188633-plasmid-map-sequence-id-369478

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  11. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  12. pX330_TUBA1B sgRNA / hSpCas9

    Plasmid
    #172834
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172834-plasmid-map-sequence-id-340790

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  15. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70707-plasmid-map-sequence-id-127770

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  18. pCMV-Csy4-PE2

    Plasmid
    #172667
    Purpose
    Enhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b
    Depositor
    Xiaolong Wang
    Publication
    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-PE2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172667-plasmid-map-sequence-id-339511

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  19. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid
    #165450
    Purpose
    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter
    Depositor
    Elvir Becirovic
    Publication
    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi
    Inserts
    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    U6 and short human rhodopsin promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165450-plasmid-map-sequence-id-331048

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Showing: 8161 - 8180 of 16006 results