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anti-PSD-95 scFv [K28/43] with sortase tag

Plasmid

#204422

Purpose

Mammalian Expression of anti-PSD-95 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K28/43.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting PSD-95 (Homo sapiens)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1delta

Plasmid

#124142

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

Plasmid

#169469

Purpose

Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/FLAG-Ino80 ΔN (267-1556)

Plasmid

#29440

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1556 (a.a.)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223_RNF212_WT

Plasmid

#81860

Purpose

Gateway Donor vector containing RNF212 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RNF212 (RNF212 Human)

Use

Gateway entry vector

Mutation

W4L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SGK1_WT

Plasmid

#81834

Purpose

Gateway Donor vector containing SGK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SGK1 (SGK1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_WT

Plasmid

#82320

Purpose

Gateway Donor vector containing STK11 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP6C_p.E180K

Plasmid

#81334

Purpose

Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP6C (PPP6C Human)

Use

Gateway entry vector

Mutation

E180K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-HRAS

Plasmid

#52730

Purpose

retroviral expression of human HRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

HRAS (HRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-CDreV

Plasmid

#140133

Purpose

can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CDreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFKBIB_WT

Plasmid

#82219

Purpose

Gateway Donor vector containing NFKBIB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFKBIB (NFKBIB Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WNT2_WT

Plasmid

#81775

Purpose

Gateway Donor vector containing WNT2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WNT2 (WNT2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-D2512H

Plasmid

#69017

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-D2512H (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Aspartate 2512 to Histidine

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Like Domain

Plasmid

#86020

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed alanine 82 to valine, threonine 230 to al…

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SV2C_WT

Plasmid

#81924

Purpose

Gateway Donor vector containing SV2C, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SV2C (SV2C Human)

Use

Gateway entry vector

Mutation

F346S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/FLAG-Ino80 ΔN-EQ (267-1556)

Plasmid

#29438

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1556 (a.a.) E653Q

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-hINO80 ΔN ΔHSA (520-1556)

Plasmid

#29446

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 520-1556 (a.a.)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pJC-elav1.8kb-TALE1-VP64-P10

Plasmid

#104609

Purpose

Expresses TALE1 under the control of a 1.8kb elav enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

1.8kb elav enhancer element (elav Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-2gamma

Plasmid

#124145

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/FLAG-Ino80 ΔNC (267-1261)

Plasmid

#29439

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1261 (a.a.) with Q1116K mutation

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only