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pcDNA-wtATMSPARK2

Plasmid

#195247

Purpose

express wtATMSPARK2

Depositor

Xiaokun Shu

Article

Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

Insert

ATMSPARK2 sensor

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

Plasmid

#71485

Purpose

Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Type

Empty backbone

Promoter

U6

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Tcf/Lef:H2B-GFP

Plasmid

#32610

Depositor

Anna-Katerina Hadjantonakis

Article

Ferrer-Vaquer et al BMC Dev Biol. 2010 Dec 21;10:121.

Insert

Tcf/Lef:H2B-GFP

Promoter

hsp68

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Article

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3-mRFP

Plasmid

#184902

Purpose

Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7, monomeric EGFP, and monomeric RFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGY199

Plasmid

#239801

Purpose

Express EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

EGFP

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDAS12069_U6-pegRNA-mCherry

Plasmid

#177180

Purpose

pegRNA cloning plasmid with mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCBL101gg

Plasmid

#199735

Purpose

Golden Gate recipient vector for transgene overexpression

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

sacB

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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ssFv_lenti_wtATMSPARK2

Plasmid

#195249

Purpose

express wtATMSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

Insert

ATMSPARK2 sensor

Use

Lentiviral

Expression

Mammalian

Promoter

ssfv

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVII

Plasmid

#162750

Purpose

MSCV backbone

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMV306hsp

Plasmid

#26155

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

hsp60 promoter

Use

Mycobacteria integrating vector

Available Since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only

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CBF:H2B-Venus

Plasmid

#44211

Depositor

Anna-Katerina Hadjantonakis

Article

Nowotschin et al BMC Dev Biol. 2013 Apr 25;13:15. doi: 10

Insert

CBF1-Responsive-Element

Expression

Mammalian

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pSiM24-eGFP

Plasmid

#67883

Purpose

Plant Gene expression Vector pSIM24 containing eGFP reporter

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Insert

eGFP

Expression

Plant

Promoter

M24

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Halo-TMD

Plasmid

#195347

Purpose

Fluorescent labeling for cell membranes

Depositor

Allen Liu

Article

Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

Insert

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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GW1CMV-Perceval

Plasmid

#21737

Purpose

can be used to monitor the ATP:ADP ratio during live-cell imaging

Depositor

Gary Yellen

Article

Berg et al Nat Methods. 2009 Feb;6(2):161-6. doi: 1

Insert

Perceval (fluorescent ATP:ADP sensor)

Tags

5'UTR

Expression

Mammalian

Mutation

improved Kozak sequence

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-GFP1

Plasmid

#106408

Purpose

P-RAN-GFP1 (RANbody GFP1::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-GFP1

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDAS12222_U6-pegRNA-BFP

Plasmid

#177181

Purpose

pegRNA cloning plasmid with BFP marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVII-LO

Plasmid

#162754

Purpose

Retroviral (MSCV) expression of LSSmOrange fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

LSSmOrange

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga1p

Plasmid

#158141

Purpose

Expresses Aga1p in yeast

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p

Expression

Yeast

Promoter

GAL10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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M-SPOTIT1.1

Plasmid

#171216

Purpose

Expresses a mu-opioid receptor-based circular permuted green fluorescent protein sensor

Depositor

Wenjing Wang

Article

Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

Insert

M-SPOTIT1.1

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-H2A2B

Plasmid

#106412

Purpose

P-RAN-H2A2B (RANbody H2A2B::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-H2A2B

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPlantPOP

Plasmid

#241283

Purpose

Cloning gene libraries from oligonucleotide pools

Depositor

Sean Cutler

Article

Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Type

Empty backbone

Use

Other

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY208

Plasmid

#239803

Purpose

Express mCherry in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

mCherry

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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K-SPOTIT1.0

Plasmid

#171215

Purpose

Expresses a kappa-opioid receptor-based circular permuted green fluorescent protein sensor

Depositor

Wenjing Wang

Article

Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

Insert

K-SPOTIT1.0

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVII-AM

Plasmid

#162751

Purpose

Retroviral (MSCV) expression of ametrine fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

ametrine

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-mutATMSPARK2

Plasmid

#195248

Purpose

express Thr to Ala mutant ATMSPARK2

Depositor

Xiaokun Shu

Article

Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

Insert

Thr to Ala mutant ATMSPARK2

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCBL102-RUBY

Plasmid

#210379

Purpose

Expresses betalain biosynthesis genes under CaMV 35S promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

35S-RUBY

Expression

Plant

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLibacceptorV2

Plasmid

#106250

Purpose

Recombination-mediated cassette exchange with target genomic loci

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCVII-tdTomato

Plasmid

#162752

Purpose

Retroviral (MSCV) expression of td Tomato fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

tdTomato

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTH101

Plasmid

#84459

Purpose

pJB38-NWMN29-30 + SarA_P1-DsRed-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-DsRED from pTH1

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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