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EK0450 SFFV-mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Plasmid

#233374

Purpose

LIDAR reporter RNA; mCherry as marker, EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM101 CMV-TO-ADAR2dd(E488Q,T501A)-MCP

Plasmid

#233432

Purpose

ADAR2dd(E488Q,T501A)-MCP (positive control that fuses ADAR2dd and MCP on the same protein)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2dd-tdMCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP(N3)-PP1beta

Plasmid

#44223

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP1B (PPP1CB Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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NbV5-LgBit

Plasmid

#201475

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-LgBit

Tags

LgBit

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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LgBit-NbV5

Plasmid

#201476

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

LgBit-NbV5

Tags

LgBit

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GFP

Plasmid

#68120

Purpose

Nuclear Localized GFP for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

GFP

Expression

Worm

Promoter

rab-3

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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809 pcDNA3 GFP PTEN

Plasmid

#10759

Depositor

William Sellers

Article

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pAR357_SFP_pCDF-1b

Plasmid

#122851

Purpose

Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

Depositor

Martin Grininger

Article

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

Sfp

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMSSM110: pCMV-HA-NLS-SpuFz1-v2

Plasmid

#205277

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

C310R/D487K/T513K

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM319 - array co-injection fragment mosTI unc-119

Plasmid

#159823

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI unc-119

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRc/RSV-m CBP-HA

Plasmid

#16701

Depositor

Richard Goodman

Article

Chrivia et al Nature. 1993 Oct 28. 365(6449):855-9.

Insert

CBP (Crebbp Mouse)

Tags

HA

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pMDJ231 - Phsp Cas9

Plasmid

#191382

Purpose

Heat shock inducible Cas9 expression in C. elegans.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Phsp-16.41 Cas9 gpd-2 tagRFP-t

Use

CRISPR

Expression

Worm

Promoter

Phsp-16.41

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

Plasmid

#205269

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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NbV5-mCherry

Plasmid

#201474

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-NRF1-HA

Plasmid

#34708

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag and HA

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Epac2

Plasmid

#118314

Purpose

Expression of mouse Epac2

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2008 Dec;28(23):7109-25.

Insert

Epac2 (Rapgef4 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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NbV5-GFP2

Plasmid

#201477

Purpose

BRET2 detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-GFP2

Tags

GFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

Plasmid

#233445

Purpose

PiggyBac donor plasmid to overexpress a full length ADAR2

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBF3038

Plasmid

#26850

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Insert

CreA recombinase

Use

Cre/Lox

Expression

Yeast

Mutation

N/A

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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