Addgene

We narrowed to 1,192 results for: 5-Oct

Showing: 801 - 820 of 1192 results

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pHAGE DN Cullin5

Plasmid

#41916

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL5 (CUL5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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bNS2B47NS3

Plasmid

#155320

Purpose

co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length

Depositor

Dahai Luo

Article

Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

Insert

Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor

Use

Tags

His tag

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES_EGFP_GA_KCNE1

Plasmid

#173160

Purpose

For mammalian expression of KCNE1 and creating variants

Depositor

Al George

Article

Kuenze et al Elife. 2020 Oct 23;9. pii: 57680. doi: 1

Insert

KCNE1 (KCNE1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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gNS2B47NS3 S135A

Plasmid

#155317

Purpose

DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutant

Depositor

Dahai Luo

Article

Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

Insert

DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor

Use

Tags

His tag

Expression

Bacterial

Mutation

S135A

Promoter

M13

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-pro-alphaf-E2-Crimson

Plasmid

#117658

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf-E2-Crimson

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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peCALWY-2

Plasmid

#22234

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-2 Zn(II) sensor (ATOX1 GFP's, Human)

Use

Tags

Expression

Mammalian

Mutation

sequence identical to eCALWY-1, but linkerlength …

Promoter

Available since

Nov. 20, 2009

Availability

Academic Institutions and Nonprofits only
pYES260-sc.eEF2

Plasmid

#85119

Purpose

yeast expression of EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/Venus-mATG5

Plasmid

#58739

Purpose

Expresses Venus-mATG5 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

Venus

Expression

Mutation

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pMRX-IP/SECFP-mATG5

Plasmid

#58744

Purpose

Expresses /SECFP-ATG5 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

SECFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pLL5.0-EGFP-HRasC20

Plasmid

#187252

Purpose

Lenticiral expression of EGFP-HRasC20

Depositor

Alpha Yap

Article

Katsuno-Kambe et al Elife. 2021 Oct 18;10. pii: 67915. doi:

Insert

HRasC20 (HRAS Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

5' LTR

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-loxP-canisNrg1

Plasmid

#173849

Purpose

Encoding dog Nrg1 before Cre-mediated recombination.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Nrg1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dEgf

Plasmid

#173700

Purpose

A knock-out vector for the dog Egf

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-neo-dTgfa

Plasmid

#173842

Purpose

A knockout vector for the dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-puro-dEgfr

Plasmid

#173844

Purpose

A knockout vector for the dog Egfr.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisEgf

Plasmid

#173845

Purpose

Encoding dog Egf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis EGF.

Use

Tags

Expression

Mammalian

Mutation

R1127Q- please see dep. comments

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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eNS2B47NS3 S135A

Plasmid

#155318

Purpose

DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutant

Depositor

Dahai Luo

Article

Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

Insert

DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor

Use

Tags

His tag

Expression

Bacterial

Mutation

S135A

Promoter

M13

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-Ost1-pro-alphaf-Btl2

Plasmid

#117665

Purpose

Study secretion efficiency of Btl2

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-pro-alphaf-Btl2

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the lipase Btl2 together wit…

Promoter

pAOX1

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYES260-sc.eEF2 K509A

Plasmid

#85120

Purpose

yeast expression of mutant EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K509A

Promoter

GAL1

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKP332 (Lenti-OSK1)

Plasmid

#21627

Depositor

Tim Townes

Article

Chang et al Stem Cells. 2009 May . 27(5):1042-9.

Insert

hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Human, Porcine teschovirus-1)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2009

Availability

Academic Institutions and Nonprofits only

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