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pLenti-VRERRA-PGK-Puro

Plasmid

#110859

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-P2A-Puro

Plasmid

#110848

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-P2A-Puro

Plasmid

#110852

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500

Plasmid

#92316

Purpose

Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

Optimized NTerm 6xHis tagged monomeric RFP (mRFP)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe mouse Bmf

Plasmid

#17241

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (Bmf Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
Strep-His-Tev-hCGN

Plasmid

#236512

Purpose

For expression of Cingulin (human) in insect cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGN (CGN Human)

Use

Tags

2xStrep-10xHis-Tev

Expression

Insect

Mutation

WT

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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mCGN-Myc-His

Plasmid

#236510

Purpose

For expression of Cingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (Cgn Mouse)

Use

Tags

Myc-His

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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hCGNL1-Myc-HA

Plasmid

#236515

Purpose

For expression of hParacingulinL1 in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGNL1 (CGNL1 Human)

Use

Tags

Myc-HA

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGN-Myc

Plasmid

#236509

Purpose

For expression of Cingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (Cgn Mouse)

Use

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGNL1-Myc

Plasmid

#236514

Purpose

For expression of Paracingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGNL1 (Cgnl1 Mouse)

Use

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGNL1-HA

Plasmid

#236517

Purpose

For expression of Paracingulin (dog) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (CGNL1 Canis lupus familiaris (dog))

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGNL1

Plasmid

#236519

Purpose

For expression of Paracingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (Cgnl1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGN-Myc

Plasmid

#236506

Purpose

For expression of CGN (dog) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGN (CGN Canis lupus familiaris (dog))

Use

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLC047 - pL2_p2x35s-CrGLK-tMAS

Plasmid

#203906

Purpose

Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Use

Tags

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Promoter

CaMV 2x35S

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSB278 - pL0_pT16H2 (pro + 5U)

Plasmid

#203889

Purpose

T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T16H2 promoter and 5'UTR

Use

Tags

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1224 - pL0_pT3R (pro + 5U)

Plasmid

#203892

Purpose

T3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T3R promoter and 5'UTR

Use

Tags

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1215 - pL0_pDAT (pro + 5U)

Plasmid

#203895

Purpose

DAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

DAT promoter and 5'UTR

Use

Tags

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSB55 - pL0_CrGLK (CDS)

Plasmid

#203903

Purpose

MoClo compatible L0 plasmid containing the CrGLK coding sequence

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Use

Tags

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-CDK5RAP2-SVA

Plasmid

#202828

Purpose

pGL3 luciferase reporter with CDK5RAP2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

intronic SVA within CDK5RAP2 gene

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-AGPAT3-SVA

Plasmid

#202827

Purpose

pGL3 luciferase reporter with AGPAT3 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

intronic SVA located in AGPAT3 gene

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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