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pEBTet-SNAP-STIL

Plasmid

#136795

Purpose

Mammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-STIL (STIL Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-HsSAS6

Plasmid

#136789

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Centrin2

Plasmid

#136784

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin2 (CETN2 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-LITAF

Plasmid

#178444

Purpose

doxycycline-inducible expression of Human LITAF in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

LITAF (LITAF Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(fl)-3

Plasmid

#27107

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

TetO promoter

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pTGV-Tet SMR5970

Plasmid

#28037

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pAN-PTet-YFP

Plasmid

#62245

Purpose

used to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Centrin3

Plasmid

#136807

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin3

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pES002 (pTet-qacR wt)

Plasmid

#69031

Purpose

wild-type qacR downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

pTet

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pFLAG-137.144S Tetherin

Plasmid

#41072

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

137S, 144S

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pXW101Plux2-32Ptet2-gfp

Plasmid

#160821

Purpose

Two-layered AHL-responsive circuit containing a TetR-based inverter

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

PJ23101-30luxR-t-Plux2-32tetR-Ptet2-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PJ23101, Plux2, Ptet2

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCIBN-TetR-tagRFP-T

Plasmid

#103809

Purpose

'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-TetR-tagRFP-T (CIB1, tetR Mustard Weed, E. coli)

Use

Tags

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-TetR

Plasmid

#103798

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-TetR (CIB1, tetR Mustard Weed, E. coli)

Use

Tags

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Nfat1

Plasmid

#139830

Purpose

doxycycline-inducible expression of mouse Nfat1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Nfat1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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TET1CD-CRY2-EGFP

Plasmid

#82555

Purpose

Encodes TET1 under LITE2.0 system

Depositor

Joseph Irudayaraj

Article

Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

Inserts

TET1 (TET1 Human)
CRY2 (CRY2 Mustard Weed)
EGFP

Use

AAV

Tags

2A, NLS(alpha-imp), and NLS-VP64

Expression

Mammalian

Mutation

CRY2PHR (N-terminal photolyase homology region do…

Promoter

EF-1alpha

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-plk4

Plasmid

#136837

Purpose

Mammalian expression of the centrosomal protein plk4 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-plk4 (PLK4 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pXW101Plux2-33Ptet2-gfp

Plasmid

#160820

Purpose

Two-layered AHL-responsive circuit containing a TetR-based inverter

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

PJ23101-30luxR-t-Plux2-33tetR-Ptet2-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PJ23101, Plux2, Ptet2

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-TetR-RNF169UBD

Plasmid

#119011

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTLR-donor-tetO

Plasmid

#119000

Purpose

Repair template for the TLR reporter with 7 tet operator elements

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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FUW TetO NET1A-V5 WT

Plasmid

#69828

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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